BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4762

Title: 15N relaxation data and model-free parameters for the N-terminal receiver domain of wild-type unphosphorylated NtrC receiver domain (NtrCr)

Authors: Kern, D.; Volkman, B.; Wemmer, D.

Citation: Volkman, Brian; Lipson, Doron; Wemmer, David; Kern, Dorothee. "Two-state allosteric behavior in a single domain signaling protein"  Science 291, 2429-2433 (2001).

Assembly members:
Nitrogen regulatory protein C N-terminal receiver domain, polymer, 124 residues, Formula weight is not available

Natural source:   Common Name: Bacteria   Taxonomy ID: 602   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Salmonella typhimurium

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Nitrogen regulatory protein C N-terminal receiver domain: MQRGIVWVVDDDSSIRWVLE RALAGAGLTCTTFENGNEVL AALASKTPDVLLSDIRMPGM DGLALLKQIKQRHPMLPVII MTAHSDLDAAVSAYQQGAFD YLPKPFDIDEAVALVERAIS HYQE

Data sets:
Data typeCount
T1 relaxation values98
T2 relaxation values196

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NITROGEN REGULATION PROTEIN1

Entities:

Entity 1, NITROGEN REGULATION PROTEIN 124 residues - Formula weight is not available

1   METGLNARGGLYILEVALTRPVALVALASP
2   ASPASPSERSERILEARGTRPVALLEUGLU
3   ARGALALEUALAGLYALAGLYLEUTHRCYS
4   THRTHRPHEGLUASNGLYASNGLUVALLEU
5   ALAALALEUALASERLYSTHRPROASPVAL
6   LEULEUSERASPILEARGMETPROGLYMET
7   ASPGLYLEUALALEULEULYSGLNILELYS
8   GLNARGHISPROMETLEUPROVALILEILE
9   METTHRALAHISSERASPLEUASPALAALA
10   VALSERALATYRGLNGLNGLYALAPHEASP
11   TYRLEUPROLYSPROPHEASPILEASPGLU
12   ALAVALALALEUVALGLUARGALAILESER
13   HISTYRGLNGLU

Samples:

sample_1: Nitrogen regulatory protein C N-terminal receiver domain, [U-99% 15N], 0.9 mM; phosphate buffer 50 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.75; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1not availablesample_cond_1

Software:

FELIX v95.0 - processing

xwinnmr v2.1 - collection

ModelFree v4.01 - data analysis

NMR spectrometers:

  • BRUKER DMX 600 MHz
  • BRUKER DMX 750 MHz

Related Database Links:

BMRB 4527 4528 4763 4764 5303
PDB
DBJ BAB38213 BAE77441 BAG79675 BAI27885 BAI33008
EMBL CAA28808 CAA59425 CAD03095 CAP78325 CAQ34219
GB AAB03002 AAC76865 AAG59057 AAL22844 AAN45373
PIR AC0950 E86074
REF NP_290493 NP_312817 NP_418304 NP_458044 NP_462885
SP P0AFB8 P0AFB9 P41789