BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4736

Title: 1H, 13C, 15N Chemical Shift Assignment for the human prion protein variant M166V

Authors: Calzolai, Luigi; Zahn, Ralph; Guntert, Peter; Wuthrich, Kurt; von Schroetter, Christine; Riek, Roland; Lysek, Dominikus

Citation: Calzolai, Luigi; Zahn, Ralph; Guntert, Peter; Wuthrich, Kurt; von Schroetter, Christine; Riek, Roland; Lysek, Dominikus. "NMR structures of three single-residue variants of the human prion protein"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:
human prion protein, polymer, 112 residues, Formula weight is not available

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
human prion protein: GSVVGGLGGYMLGSAMSRPI IHFGSDYEDRYYRENMHRYP NQVYYRPVDEYSNQNNFVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCITQY ERESQAYYQRGS

Data sets:
Data typeCount
1H chemical shifts770
13C chemical shifts355
15N chemical shifts126

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1hPrP1

Entities:

Entity 1, hPrP 112 residues - Formula weight is not available

1   GLYSERVALVALGLYGLYLEUGLYGLYTYR
2   METLEUGLYSERALAMETSERARGPROILE
3   ILEHISPHEGLYSERASPTYRGLUASPARG
4   TYRTYRARGGLUASNMETHISARGTYRPRO
5   ASNGLNVALTYRTYRARGPROVALASPGLU
6   TYRSERASNGLNASNASNPHEVALHISASP
7   CYSVALASNILETHRILELYSGLNHISTHR
8   VALTHRTHRTHRTHRLYSGLYGLUASNPHE
9   THRGLUTHRASPVALLYSMETMETGLUARG
10   VALVALGLUGLNMETCYSILETHRGLNTYR
11   GLUARGGLUSERGLNALATYRTYRGLNARG
12   GLYSER

Samples:

sample_1: human prion protein, [U-95% 13C; U-95% 15N], 1.2 mM

Ex-cond_1: pH: 4.5; temperature: 293 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 750 MHz

Related Database Links:

BMRB 16743 16757 17714 17756 17757 17780 18426 18550 19268 4379 4402 4434 4620 4641
PDB
DBJ BAA00011 BAF62360 BAG32276 BAG32277 BAG32278
EMBL CAA58442 CAG46836 CAG46869
GB AAA19664 AAA60182 AAA68632 AAA68633 AAB59442
REF NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP P04156 P40252 P61766 P61767 P61768