BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4727

Title: Another piece of the Ribosome: Solution Structure of S16 and its Location in the 30S Subunit

Authors: Allard, Peter; Rak, A.; Wimberly, B.; Clemons, W.; Kalinin, A.; Helgstrand, M.; Garber, M.; Ramakrishnan, V.; Hard, T.

Citation: Allard, Peter; Rak, A.; Wimberly, B.; Clemons, W.; Kalinin, A.; Helgstrand, M.; Garber, M.; Ramakrishnan, V.; Hard, T.. "Another piece of the Ribosome: Solution Structure of S16 and its Location in the 30S Subunit"  Structure Fold. Des. 8, 875-882 (2000).

Assembly members:
S16, polymer, 88 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: 274   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Thermus thermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
S16: MVKIRLARFGSKHNPHYRIV VTDARRKRDGKYIEKIGYYD PRKTTPDWLKVDVERARYWL SVGAQPTDTARRLLRQAGVF RQEAREGA

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts83
1H chemical shifts627

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S161

Entities:

Entity 1, S16 88 residues - Formula weight is not available

1   METVALLYSILEARGLEUALAARGPHEGLY
2   SERLYSHISASNPROHISTYRARGILEVAL
3   VALTHRASPALAARGARGLYSARGASPGLY
4   LYSTYRILEGLULYSILEGLYTYRTYRASP
5   PROARGLYSTHRTHRPROASPTRPLEULYS
6   VALASPVALGLUARGALAARGTYRTRPLEU
7   SERVALGLYALAGLNPROTHRASPTHRALA
8   ARGARGLEULEUARGGLNALAGLYVALPHE
9   ARGGLNGLUALAARGGLUGLYALA

Samples:

sample_1: S16 1.5 mM

sample_2: S16, [U-100% 13C; U-90% 15N], 1.5 mM

cond_1: ionic strength: 0.2 M; pH*: 6.0; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
DQF-COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
1H-15N NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
(HB)CB(CGCD)HDnot availablenot availablenot available
(HB)CB(CGCDCE)HEnot availablenot availablenot available

Software:

ANSIG v3.3 - assignment

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAD70858
GB AAB50700 AAS81021 AEG33456 AFH38923
REF WP_011173115 YP_144301
SP P62238 Q5SJH3