BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4678

Title: Solution Structure of the RNA Polymerase Subunit RPB5 from Methanobacterium thermoautotrophicum

Authors: Yee, Adelinda; Booth, Valerie; Dharamsi, Akil; Engel, Asaph; Edwards, Aled; Arrowsmith, Cheryl

Citation: Yee, Adelinda; Booth, Valerie; Dharamsi, Akil; Engel, Asaph; Edwards, Aled; Arrowsmith, Cheryl. "Solution Structure of the RNA Polymerase Subunit RPB5 from Methanobacterium thermoautotrophicum"  Proc. Natl. Acad. Sci. U. S. A. 97, 6311-6315 (2000).

Assembly members:
rna polymerase subunit 5, polymer, 77 residues, Formula weight is not available

Natural source:   Common Name: Methanobacterium thermoautotrophicum   Taxonomy ID: 2166   Superkingdom: Archaea   Kingdom: not available   Genus/species: Methanobacterium thermoautotrophicum

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
rna polymerase subunit 5: MKREILKHQLVPEHVILNES EAKRVLKELDAHPEQLPKIK TTDPVAKAIGAKRGDIVKII RKSPTAEEFVTYRLVQD

Data sets:
Data typeCount
1H chemical shifts566
13C chemical shifts269
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rpb51

Entities:

Entity 1, rpb5 77 residues - Formula weight is not available

1   METLYSARGGLUILELEULYSHISGLNLEU
2   VALPROGLUHISVALILELEUASNGLUSER
3   GLUALALYSARGVALLEULYSGLULEUASP
4   ALAHISPROGLUGLNLEUPROLYSILELYS
5   THRTHRASPPROVALALALYSALAILEGLY
6   ALALYSARGGLYASPILEVALLYSILEILE
7   ARGLYSSERPROTHRALAGLUGLUPHEVAL
8   THRTYRARGLEUVALGLNASP

Samples:

sample_one: rna polymerase subunit 5, [U-13C; U-15N], 1 mM

sample_conditions_one: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCACBsample_onenot availablesample_conditions_one
HBCBCACONNHsample_onenot availablesample_conditions_one
HCCHTOCSYsample_onenot availablesample_conditions_one
HCCTOCSYsample_onenot availablesample_conditions_one
HNCOsample_onenot availablesample_conditions_one
CCC-TOCSYsample_onenot availablesample_conditions_one
15N-DIPSIsample_onenot availablesample_conditions_one

Software:

nmrview v3.0 - peak picking

NMR spectrometers:

  • Varian Inova 500 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAM70200
GB AAB85539
REF WP_010876674 WP_048175656
SP O27122