BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4673

Title: Solution structure of Ileal Lipid Binding Protein in complex with glycocholate

Deposition date: 2000-02-26 Original release date: 2000-05-24

Authors: Luecke, Christian; Zhang, Fengli; Hamilton, James; Sacchettini, James; Rueterjans, Heinz

Citation: Luecke, Christian; Zhang, Fengli; Hamilton, James; Sacchettini, James; Rueterjans, Heinz. "Solution structure of Ileal Lipid Binding Protein in complex with glycocholate"  Eur. J. Biochem. 267, 2929-2938 (2000).

Assembly members:
ileal lipid binding protein, polymer, 127 residues, 14088 Da.
GCH, non-polymer, 465.623 Da.

Natural source:   Common Name: pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ileal lipid binding protein: AFTGKYEIESEKNYDEFMKR LALPSDAIDKARNLKIISEV KQDGQNFTWSQQYPGGHSIT NTFTIGKECDIETIGGKKFK ATVQMEGGKVVVNSPNYHHT AEIVDGKLVEVSTVGGVSYE RVSKKLA

Data sets:
Data typeCount
1H chemical shifts901

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1ILBP1
2glycocholate2

Entities:

Entity 1, ILBP 127 residues - 14088 Da.

1   ALAPHETHRGLYLYSTYRGLUILEGLUSER
2   GLULYSASNTYRASPGLUPHEMETLYSARG
3   LEUALALEUPROSERASPALAILEASPLYS
4   ALAARGASNLEULYSILEILESERGLUVAL
5   LYSGLNASPGLYGLNASNPHETHRTRPSER
6   GLNGLNTYRPROGLYGLYHISSERILETHR
7   ASNTHRPHETHRILEGLYLYSGLUCYSASP
8   ILEGLUTHRILEGLYGLYLYSLYSPHELYS
9   ALATHRVALGLNMETGLUGLYGLYLYSVAL
10   VALVALASNSERPROASNTYRHISHISTHR
11   ALAGLUILEVALASPGLYLYSLEUVALGLU
12   VALSERTHRVALGLYGLYVALSERTYRGLU
13   ARGVALSERLYSLYSLEUALA

Entity 2, glycocholate - C26 H43 N O6 - 465.623 Da.

1   GCH

Samples:

sample_1: ileal lipid binding protein3 – 4 mM

cond1: pH: 5.0; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYsample_1not availablecond1
1H-1H NOESYsample_1not availablecond1
1H-13C NOESYsample_1not availablecond1

Software:

XWINNMR v1.3 - data acquisition and processing

AURELIA v2.1 - data analysis

FELIX v95.0 - data analysis

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
GB AAA77657
REF NP_999380
SP P10289