BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4632

Title: Solution Structure of a Pro-Apoptotic Protein BAX   PubMed: 11106734

Authors: Suzuki, M.; Hsu, Y.-T.; Youle, R.; Tjandra, N.

Citation: Suzuki, M.; Hsu, Y.-T.; Youle, R.; Tjandra, N.. "Structure of Bax: Co-Regulation of Dimer Formation and Intracellular Localization "  Cell 103, 645-654 (2000).

Assembly members:
PROTEIN (APOPTOSIS REGULATOR BAX, MEMBRANE ISOFORM ALPHA), polymer, 192 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PROTEIN (APOPTOSIS REGULATOR BAX, MEMBRANE ISOFORM ALPHA): MDGSGEQPRGGGPTSSEQIM KTGALLLQGFIQDRAGRMGG EAPELALDPVPQDASTKKLS ECLKRIGDELDSNMELQRMI AAVDTDSPREVFFRVAADMF SDGNFNWGRVVALFYFASKL VLKALCTKVPELIRTIMGWT LDFLRERLLGWIQDQGGWDG LLSYFGTPTWQTVTIFVAGV LTASLTIWKKMG

Data sets:
Data typeCount
residual dipolar couplings604

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BAX1

Entities:

Entity 1, BAX 192 residues - Formula weight is not available

1   METASPGLYSERGLYGLUGLNPROARGGLY
2   GLYGLYPROTHRSERSERGLUGLNILEMET
3   LYSTHRGLYALALEULEULEUGLNGLYPHE
4   ILEGLNASPARGALAGLYARGMETGLYGLY
5   GLUALAPROGLULEUALALEUASPPROVAL
6   PROGLNASPALASERTHRLYSLYSLEUSER
7   GLUCYSLEULYSARGILEGLYASPGLULEU
8   ASPSERASNMETGLULEUGLNARGMETILE
9   ALAALAVALASPTHRASPSERPROARGGLU
10   VALPHEPHEARGVALALAALAASPMETPHE
11   SERASPGLYASNPHEASNTRPGLYARGVAL
12   VALALALEUPHETYRPHEALASERLYSLEU
13   VALLEULYSALALEUCYSTHRLYSVALPRO
14   GLULEUILEARGTHRILEMETGLYTRPTHR
15   LEUASPPHELEUARGGLUARGLEULEUGLY
16   TRPILEGLNASPGLNGLYGLYTRPASPGLY
17   LEULEUSERTYRPHEGLYTHRPROTHRTRP
18   GLNTHRVALTHRILEPHEVALALAGLYVAL
19   LEUTHRALASERLEUTHRILETRPLYSLYS
20   METGLY

Samples:

sample_1: PROTEIN (APOPTOSIS REGULATOR BAX, MEMBRANE ISOFORM ALPHA), [U-15N; U-13C], 1 mM; tris-acetate 10 mM; DTT 2 mM

sample_cond_1: pH: 6.00; temperature: 305.00 K; ionic strength: 0.01 M; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
4D 13C/15N-SEPARATED NOESYsample_1not availablesample_cond_1
4D 13C-SEPARATED NOESYsample_1not availablesample_cond_1
3D 15N-SEPARATED NOESYsample_1not availablesample_cond_1

Software:

X-PLOR v3.840 - refinement

NMRPIPE v1.7 REV (1999.039.11.31) - structure solution

PIPP v4.1.5 - structure solution

UXNMR v2.5 - structure solution

NMR spectrometers:

  • BRUKER DRX 800 MHz

Related Database Links:

BMRB 18340
PDB
DBJ BAC53619 BAF83765 BAI46883
EMBL CAD10744 CCF78739
GB AAA03619 AAA03620 AAC48806 AAD22706 AAF82094
REF NP_001003011 NP_001247945 NP_001278357 NP_001278358 NP_001278360
SP O02703 Q07812
TPG DAA19512