BMRB Entry 4619
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4619
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Eijkelenboom, A.; van den Ent, F.; Vos, A.; Doreleijers, J.; Hard, K.; Tullius, T.; Plasterk, R.; Kaptein, R.; Boelens, R.. "Solution structure of the N-terminal HHCC domain of HIV-2 integrase: a
three-helix bundle stabilized by zinc" Curr. Biol. 7, 739-746 (1997).
Assembly members:
Human immunodeficiency virus type 2 integrase, polymer, 55 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: HIV-2 Taxonomy ID: 11720 Superkingdom: not available Kingdom: not available Genus/species: not available Human immunodeficiency virus type 2
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)
Entity Sequences (FASTA):
Human immunodeficiency virus type 2 integrase: FLEKIEPAQEEHEKYHSNVK
ELSHKFGIPNLVARQIVNSC
AQCQQKGEAIHGQVN
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 398 |
| 15N chemical shifts | 62 |
| 13C chemical shifts | 184 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HHCC domain of HIV-2 monomer 1 | 1 |
| 2 | HHCC domain of HIV-2 monomer 2 | 1 |
| 3 | Zn-1 | 2 |
| 4 | Zn-2 | 2 |
Entities:
Entity 1, HHCC domain of HIV-2 monomer 1 55 residues - Formula weight is not available
| 1 | PHE | LEU | GLU | LYS | ILE | GLU | PRO | ALA | GLN | GLU | ||||
| 2 | GLU | HIS | GLU | LYS | TYR | HIS | SER | ASN | VAL | LYS | ||||
| 3 | GLU | LEU | SER | HIS | LYS | PHE | GLY | ILE | PRO | ASN | ||||
| 4 | LEU | VAL | ALA | ARG | GLN | ILE | VAL | ASN | SER | CYS | ||||
| 5 | ALA | GLN | CYS | GLN | GLN | LYS | GLY | GLU | ALA | ILE | ||||
| 6 | HIS | GLY | GLN | VAL | ASN |
Entity 2, Zn-1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Human immunodeficiency virus type 2 integrase, [U-15N], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; D2O 99.99%
sample_2: Human immunodeficiency virus type 2 integrase, [U-15N], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; H2O 95%; D2O 5%
sample_3: Human immunodeficiency virus type 2 integrase, [U-15N; U-13C], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; D2O 5%
sample_4: Human immunodeficiency virus type 2 integrase, [U-15N; U-13C], 3.5 mM; ZINC (II) ION 1.1 eq; Tris, [U-2H], 50 mM; NaCl 150 mM; 2-mercaptoethanol 2 mM; H2O 95%; D2O 5%
sample_cond_1: pH: 6.5; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D (1H,15N)-HSQC | not available | not available | sample_cond_1 |
| 2D (1H,15N)-HSMQC | not available | not available | sample_cond_1 |
| 2D (1H,1H) NOESY | not available | not available | sample_cond_1 |
| 2D (1H,1H) TOCSY | not available | not available | sample_cond_1 |
| 3D TOCSY (15N,1H) HSQC | not available | not available | sample_cond_1 |
| 3D TOCSY (13C,1H) HSQC | not available | not available | sample_cond_1 |
| 3D NOESY (15N,1H) HSQC | not available | not available | sample_cond_1 |
| 3D NOESY (13C,1H) HSQC | not available | not available | sample_cond_1 |
| 3D (13C) HMQC NOESY (13C,1H) HSQC | not available | not available | sample_cond_1 |
| 2D 13C-filtered NOESY | not available | not available | sample_cond_1 |
| 3D 13C-filtered NOESY (13C,1H) HSQC | not available | not available | sample_cond_1 |
| 3D HNCO | not available | not available | sample_cond_1 |
| 3D HNCA | not available | not available | sample_cond_1 |
| 3D HN(CO)CA | not available | not available | sample_cond_1 |
| 3D HNHA | not available | not available | sample_cond_1 |
| 3D HNHB | not available | not available | sample_cond_1 |
| 3D H(C)CH DIPSI | not available | not available | sample_cond_1 |
| 3D HCC(H) DIPSI | not available | not available | sample_cond_1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity Plus 500 MHz
- Bruker AMX 600 MHz
- Varian Unity Plus 750 MHz
Related Database Links:
| PDB | |
| EMBL | CAA08005 CAA08033 CAA08041 |
| GB | AAB00764 ABU88863 ADQ26695 AEI83209 AHI42063 |
| PRF | 1306388B |
| SP | P04584 |
| AlphaFold | P04584 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks