BMRB Entry 4602

Title:
Solution Structure of Oxidized Microsomal Rabbit Cytochrome b5. Factors Determining the Heterogeneous Binding of the Heme
Deposition date:
2000-05-05
Original release date:
2000-12-06
Authors:
Banci, L.; Bertini, I.; Rosato, A.; Scacchieri, S.
Citation:

Citation: Banci, L.; Bertini, I.; Rosato, A.; Scacchieri, S.. "Solution structure of oxidized microsomal rabbit cytochrome b5. Factors determining the heterogeneous binding of the heme"  Eur. J. Biochem. 267, 755-766 (2000).
PubMed: 10651812

Assembly members:

Assembly members:
CYTOCHROME B5, polymer, 94 residues, Formula weight is not available
HEM, non-polymer, 616.487 Da.

Natural source:

Natural source:   Common Name: European Rabbit   Taxonomy ID: 9986   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Oryctolagus cuniculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK223

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts538
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CYTOCHROME B51
2heme2

Entities:

Entity 1, CYTOCHROME B5 94 residues - Formula weight is not available

1   ASPLYSASPVALLYSTYRTYRTHRLEUGLU
2   GLUILELYSLYSHISASNHISSERLYSSER
3   THRTRPLEUILELEUHISHISLYSVALTYR
4   ASPLEUTHRLYSPHELEUGLUGLUHISPRO
5   GLYGLYGLUGLUVALLEUARGGLUGLNALA
6   GLYGLYASPALATHRGLUASNPHEGLUASP
7   VALGLYHISSERTHRASPALAARGGLULEU
8   SERLYSTHRPHEILEILEGLYGLULEUHIS
9   PROASPASPARGSERLYSLEUSERLYSPRO
10   METGLUTHRLEU

Entity 2, heme - C34 H32 Fe N4 O4 - 616.487 Da.

1   HEM

Samples:

sample_1: CYTOCHROME B5, [U-15N], 2 – 3 mM; phosphate buffer 1 mM

sample_cond_1: pH: 7; temperature: 298 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D NOESYsample_1not availablesample_cond_1
3D 15N-SEPARATED_NOESYsample_1not availablesample_cond_1
2D TOCSYsample_1not availablesample_cond_1
3D 15N-SEPARATED_TOCSYsample_1not availablesample_cond_1

Software:

DYANA v1.5 MODIFIED FOR USING PSEUDOCONTACT SHIFTS AS CONSTRAINTS - STRUCTURE SOLUTION

AMBER v4.0 - REFINEMENT

NMR spectrometers:

  • Bruker Avance 800 MHz

Related Database Links:

BMRB 18919 295
PDB
DBJ BAA01712
GB AAB03878 AAB32285
PRF 1205244A 1908210A
REF NP_001164734 NP_001164735
SP P00169
AlphaFold P00169

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks