BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4577

Title: 1H, 13C, and 15N Chemical Shift Assignments for Ribosomal Protein S4 delta 41

Authors: Markus, Michelle; Gerstner, Resi; Draper, David; Torchia, Dennis

Citation: Markus, Michelle; Gerstner, Resi; Draper, David; Torchia, Dennis. "The solution structure of ribosomal protein S4 delta41 reveals two subdomains and a positively charged surface that may interact with RNA."  EMBO J. 17, 4559-4571 (1998).

Assembly members:
RIBOSOMAL PROTEIN S4 DELTA 41, polymer, 159 residues, 18595 Da.

Natural source:   Common Name: Bacteria   Taxonomy ID: 1422   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus stearothermophilus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
RIBOSOMAL PROTEIN S4 DELTA 41: MKLSEYGLQLQEKQKLRHMY GVNERQFRKTFEEAGKMPGK HGENFMILLESRLDNLVYRL GLARTRRQARQLVTHGHILV DGSRVNIPSYRVKPGQTIAV REKSRNLQVIKEALEANNYI PDYLSFDPEKMEGTYTRLPE RSELPAEINEALIVEFYSR

Data sets:
Data typeCount
1H chemical shifts1080
13C chemical shifts712
15N chemical shifts173
coupling constants113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S4D411

Entities:

Entity 1, S4D41 159 residues - 18595 Da.

1   METLYSLEUSERGLUTYRGLYLEUGLNLEU
2   GLNGLULYSGLNLYSLEUARGHISMETTYR
3   GLYVALASNGLUARGGLNPHEARGLYSTHR
4   PHEGLUGLUALAGLYLYSMETPROGLYLYS
5   HISGLYGLUASNPHEMETILELEULEUGLU
6   SERARGLEUASPASNLEUVALTYRARGLEU
7   GLYLEUALAARGTHRARGARGGLNALAARG
8   GLNLEUVALTHRHISGLYHISILELEUVAL
9   ASPGLYSERARGVALASNILEPROSERTYR
10   ARGVALLYSPROGLYGLNTHRILEALAVAL
11   ARGGLULYSSERARGASNLEUGLNVALILE
12   LYSGLUALALEUGLUALAASNASNTYRILE
13   PROASPTYRLEUSERPHEASPPROGLULYS
14   METGLUGLYTHRTYRTHRARGLEUPROGLU
15   ARGSERGLULEUPROALAGLUILEASNGLU
16   ALALEUILEVALGLUPHETYRSERARG

Samples:

15N_sample: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N], 0.800 mM; deuterated acetate 20 mM; KCl 250 mM; sodium azide 0.1 mM

15N13C_sample_1: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N; U-13C], 1.1 mM; D2O 6%; deuterated acetate 20 mM; KCl 250 mM; sodium azide 0.1 mM

15N13C_sample_2: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N; U-13C], 1.1 mM; D2O 99.9%; deuterated acetate 20 mM; KCl 250 mM; sodium azide 0.1 mM

bicelle_sample: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N], 0.100 mM; 3:1 DMPC:DHPC n/a; KH2PO4 10 mM; sodium azide 0.1 mM; D2O 7%

Pf1_sample: RIBOSOMAL PROTEIN S4 DELTA 41, [U-15N; U-85% 2H], [U-13C]-MET, [13C, CE]-TYR, 0.214 mM; Pf1 phage 2.5 mg/ml; KH2PO4 10 mM; KCl 300 mM; sodium azide 0.2 mM; D2O 6%

sample_cond_1: pH: 5.4; temperature: 310 K; pressure: 1 atm

sample_cond_2: pH: 6.1; temperature: 310 K; pressure: 1 atm

sample_cond_3: pH: 6.5; temperature: 310 K; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCAnot availablenot availablenot available
HCACOnot availablenot availablenot available
HNCOnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HNHBnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
3D_15N-separated_NOESYnot availablenot availablenot available
3D_13C-separated_NOESYnot availablenot availablenot available
4D_13C-separated_NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
IPAP 15N HSQCnot availablenot availablenot available

Software:

XWINNMR v2.5 - spectrometer operation and preliminary processing

NMRPIPE v1.7 - processing spectra

PIPP v4.2.8 - spectral analysis

X-PLOR v3.8, 4.0 - solving the structure

NMR spectrometers:

  • Bruker DMX 500 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAD77087 GAD11883 GAJ57797
GB AAB21091 ACX77386 ADI25738 ADU95212 AEV20455
REF WP_011232276 WP_013144461 WP_025949527 WP_031407624 WP_033014494
SP P81288 Q5KW49