BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4572

Title: Conformational changes in the PBX Homeodomain and C-terminal Extension upon Binding DNA and HOX-derived YPWM Peptides

Authors: Sprules, Tara; Green, Nancy; Featherstone, Mark; Gehring, Kalle

Citation: Sprules, Tara; Green, Nancy; Featherstone, Mark; Gehring, Kalle. "Conformational changes in the PBX Homeodomain and C-terminal Extension upon Binding DNA and HOX-derived YPWM Peptides"  Biochemistry 39, 9943-9950 (2000).

Assembly members:
PBX1 homeodomain, polymer, 75 residues, 8874 Da.

Natural source:   Common Name: mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
PBX1 homeodomain: GHHHHHHHHHHSSGHIEGRH MNKQATEILNEYFYSHLSNP YPSEEAKEELAKKCGITVSQ VSNWFGNKRIRYKKN

Data sets:
  • assigned_chemical_shifts
Data typeCount
1H chemical shifts448
13C chemical shifts244
15N chemical shifts72

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PBX HD1

Entities:

Entity 1, PBX HD 75 residues - 8874 Da.

1   GLYHISHISHISHISHISHISHISHISHIS
2   HISSERSERGLYHISILEGLUGLYARGHIS
3   METASNLYSGLNALATHRGLUILELEUASN
4   GLUTYRPHETYRSERHISLEUSERASNPRO
5   TYRPROSERGLUGLUALALYSGLUGLULEU
6   ALALYSLYSCYSGLYILETHRVALSERGLN
7   VALSERASNTRPPHEGLYASNLYSARGILE
8   ARGTYRLYSLYSASN

Samples:

sample_1: PBX1 homeodomain, [U-13C; U-15N], 1.0 mM; sodium phosphate 20 mM

condition_1: pH: 4.8; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
15N HMQC NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-1H COSYnot availablenot availablenot available
13C HSQCnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCOnot availablenot availablenot available
TOCSY(CO)NHnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GB AAP95000 AAP95001 AAP95002 AAP95003 AAP95004