BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4463

Title: Sequential NMR assignment of the Ras-binding domain of Byr2

Authors: Huber, Fritz; Gronwald, Wolfram; Wohlgemuth, Sabine; Herrmann, Christian; Geyer, Matthias; Wittinghofer, Alfred; Kalbitzer, Hans

Citation: Huber, Fritz; Gronwald, Wolfram; Wohlgemuth, Sabine; Herrmann, Christian; Geyer, Matthias; Wittinghofer, Alfred; Kalbitzer, Hans. "Letter to the Editor: Sequential NMR Assignment of the RAS-binding Domain of Byr2"  J. Biomol. NMR 16, 355-356 (2000).

Assembly members:
Byr2, polymer, 116 residues, 13193 Da.

Natural source:   Common Name: fission yeast   Taxonomy ID: 4896   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Schizosaccharomyces pombe

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Byr2: REFPRPCILRFIACNGQTRA VQSRGDYQKTLAIALKKFSL EDASKFIVCVSQSSRIKLIT EEEFKQICFNSSSPERDRLI IVPKEKPCPSFEDLRRSWEI ELAQPAALSSQSSLSP

Data sets:
Data typeCount
1H chemical shifts584
13C chemical shifts217
15N chemical shifts110

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Byr21

Entities:

Entity 1, Byr2 116 residues - 13193 Da.

1   ARGGLUPHEPROARGPROCYSILELEUARG
2   PHEILEALACYSASNGLYGLNTHRARGALA
3   VALGLNSERARGGLYASPTYRGLNLYSTHR
4   LEUALAILEALALEULYSLYSPHESERLEU
5   GLUASPALASERLYSPHEILEVALCYSVAL
6   SERGLNSERSERARGILELYSLEUILETHR
7   GLUGLUGLUPHELYSGLNILECYSPHEASN
8   SERSERSERPROGLUARGASPARGLEUILE
9   ILEVALPROLYSGLULYSPROCYSPROSER
10   PHEGLUASPLEUARGARGSERTRPGLUILE
11   GLULEUALAGLNPROALAALALEUSERSER
12   GLNSERSERLEUSERPRO

Samples:

sample_1: Byr2, [U-13C; U-15N], 0.7 mM; phosphate buffer 20 mM; DTE 25 mM; glycine, [U-2H], 0.200 M

sample_2: Byr2, [U-15N], 1.2 mM

sample_3: Byr2 1.2 mM

sample_1_conditions: pH: 6.9; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
CBCA(CO)NHnot availablenot availablesample_1_conditions
CBCANHnot availablenot availablesample_1_conditions
HBHA(CO)NHnot availablenot availablesample_1_conditions
HNCAnot availablenot availablesample_1_conditions
HN(CO)CAnot availablenot availablesample_1_conditions
TOCSY-HSQCnot availablenot availablesample_1_conditions
NOESY-HSQCnot availablenot availablesample_1_conditions
HCCH-TOCSYnot availablenot availablesample_1_conditions
HNHAnot availablenot availablesample_1_conditions
2D NOESY and TOCSY spectra measured in H2Onot availablenot availablesample_1_conditions
and D2O.not availablenot availablesample_1_conditions
2D 1H-15N and 1H-13C HSQCnot availablenot availablesample_1_conditions

Software:

XWINNMR v2.5 - aquisition and processing of spectra

AURELIA v2.5 - sequence specific assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 800 MHz

Related Database Links:

PDB
EMBL CAA48731 CAB10150
GB AAA35289
REF NP_595714
SP P28829