BMRB Entry 4455

Title:
Glycan-free mutant adhesion domain of human CD58 (LFA-3)
Deposition date:
1999-11-11
Original release date:
1999-12-22
Authors:
Sun, Z. Y.; Dotsch, V.; Kim, M.; Li, J.; Reinherz, E.; Wagner, G.
Citation:

Citation: Sun, Z. Y.; Dotsch, V.; Kim, M.; Li, J.; Reinherz, E.; Wagner, G.. "Functional Glycan-free Adhesion Domain of Human Cell Surface Receptor CD58: Design, Production, and NMR Studies"  EMBO J. 18, 2941-2949 (1999).

Assembly members:

Assembly members:
1dCD58, polymer, 95 residues, 11200 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Data sets:
Data typeCount
13C chemical shifts375
15N chemical shifts97
1H chemical shifts603

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
11dCD581

Entities:

Entity 1, 1dCD58 95 residues - 11200 Da.

1   SERSERGLNGLNILETYRGLYVALLYSTYR
2   GLYASNVALTHRPHEHISVALPROSERASN
3   GLNPROLEULYSGLUVALLEUTRPLYSLYS
4   GLNLYSASPLYSVALALAGLULEUGLUASN
5   SERGLUPHEARGALAPHESERSERPHELYS
6   ASNARGVALTYRLEUASPTHRLYSSERGLY
7   SERLEUTHRILETYRASNLEUTHRSERSER
8   ASPGLUASPGLUTYRGLUMETGLUSERPRO
9   ASNILETHRASPSERMETLYSPHEPHELEU
10   TYRVALGLYGLUSER

Samples:

sample_1: 1dCD58, [U-13C; U-15N], 0.2 – 0.6 mM; NaPO4 10 mM; D2O 10%

sample_cond_1: ionic strength: 0.01 M; pH: 7.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
NOESYsample_1not availablesample_cond_1
NOESY-HSQCsample_1not availablesample_cond_1
HNCAsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1
HBHA(CBCACO)NHsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
HNHBsample_1not availablesample_cond_1
HNCOsample_1not availablesample_cond_1
HN(CA)COsample_1not availablesample_cond_1

Software:

XEASY -

DIANA -

DYANA -

XPLOR -

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Varian UnityInova 500 MHz
  • Varian UnityInova 750 MHz

Related Database Links:

PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks