BMRB Entry 4438

Title:
1H, 13C and 15N chemical Shift Assignments for SCP2
Deposition date:
1999-10-14
Original release date:
2000-03-23
Authors:
Lopez Garcia, Francisco; Szyperski, Thomas; Dyer, James; Choinowski, Thomas; Seedorf, Udo; Hauser, Helmut; Wuthrich, Kurt
Citation:

Citation: Lopez Garcia, Francisco; Szyperski, Thomas; Dyer, James; Choinowski, Thomas; Seedorf, Udo; Hauser, Helmut; Wuthrich, Kurt. "NMR structure of the sterol carrier protein-2; implications for the biological role"  J. Mol. Biol. 295, 595-603 (2000).
PubMed: 10623549

Assembly members:

Assembly members:
Sterol carrier protein 2, polymer, 123 residues, 13242.3 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-2T/hSCP2

Data sets:
Data typeCount
1H chemical shifts755
13C chemical shifts301
15N chemical shifts124

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SCP-21

Entities:

Entity 1, SCP-2 123 residues - 13242.3 Da.

1   SERSERALASERASPGLYPHELYSALAASN
2   LEUVALPHELYSGLUILEGLULYSLYSLEU
3   GLUGLUGLUGLYGLUGLNPHEVALLYSLYS
4   ILEGLYGLYILEPHEALAPHELYSVALLYS
5   ASPGLYPROGLYGLYLYSGLUALATHRTRP
6   VALVALASPVALLYSASNGLYLYSGLYSER
7   VALLEUPROASNSERASPLYSLYSALAASP
8   CYSTHRILETHRMETALAASPSERASPPHE
9   LEUALALEUMETTHRGLYLYSMETASNPRO
10   GLNSERALAPHEPHEGLNGLYLYSLEULYS
11   ILETHRGLYASNMETGLYLEUALAMETLYS
12   LEUGLNASNLEUGLNLEUGLNPROGLYASN
13   ALALYSLEU

Samples:

sample1: Sterol carrier protein 2, [U-15N], 1.0 mM

sample2: Sterol carrier protein 2, [U-13C; U-15N], 1 mM

conditions_set1: pH: 6.0; temperature: 309 K; ionic strength: 0.45 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availableconditions_set1
1H-15N TOCSYnot availablenot availableconditions_set1
HNCACBCA(CO)NHnot availablenot availableconditions_set1
1H-13C-NOESYnot availablenot availableconditions_set1
HCCH-COSYnot availablenot availableconditions_set1
four channels, Z-gradient, triple resonance probenot availablenot availableconditions_set1

Software:

PROSA v4.3 - Processing of NMR data

XEASY - NMR spectral analysis

DYANA v1.5 - Structure calculation, Torsion angle dynamics

NMR spectrometers:

  • Varian Unity+ 750 MHz
  • Bruker AMX 600 MHz

Related Database Links:

PDB
DBJ BAG35708 BAG57810 BAG58208 BAG64455
EMBL CAH91926
GB AAA03557 AAA03558 AAA03559 AAB41286 AAH05911
REF NP_001007100 NP_001007101 NP_001126116 NP_001180528 NP_001180529
SP P22307
AlphaFold P22307

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks