BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4431

Title: Structure of the Soluble Methane Monooxygenase Regulatory Protein B

Authors: Walters, K.; Gassner, G.; Lippard, S.; Wagner, G.

Citation: Walters, K.; Gassner, G.; Lippard, S.; Wagner, G.. "Structure of the soluble methane monooxygenase regulatory protein B"  Proc. Natl. Acad. Sci. U. S. A. 96, 7877-7882 (1999).

Assembly members:
methane monooxygenase regulatory protein B, polymer, 141 residues, Formula weight is not available

Natural source:   Common Name: Methylococcus capsulatus   Taxonomy ID: 414   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Methylococcus capsulatus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
methane monooxygenase regulatory protein B: MSVNSNAYDAGIMGLKGKDF ADQFFADENQVVHESDTVVL VLKKSDEINTFIEEILLTDY KKNVNPTVNVEDRAGYWWIK ANGKIEVDCDEISELLGRQF NVYDFLVDVSSTIGRAYTLG NKFTITSELMGLDRKLEDYH A

Data sets:
Data typeCount
1H chemical shifts794
13C chemical shifts241
15N chemical shifts151

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MMOB1

Entities:

Entity 1, MMOB 141 residues - Formula weight is not available

1   METSERVALASNSERASNALATYRASPALA
2   GLYILEMETGLYLEULYSGLYLYSASPPHE
3   ALAASPGLNPHEPHEALAASPGLUASNGLN
4   VALVALHISGLUSERASPTHRVALVALLEU
5   VALLEULYSLYSSERASPGLUILEASNTHR
6   PHEILEGLUGLUILELEULEUTHRASPTYR
7   LYSLYSASNVALASNPROTHRVALASNVAL
8   GLUASPARGALAGLYTYRTRPTRPILELYS
9   ALAASNGLYLYSILEGLUVALASPCYSASP
10   GLUILESERGLULEULEUGLYARGGLNPHE
11   ASNVALTYRASPPHELEUVALASPVALSER
12   SERTHRILEGLYARGALATYRTHRLEUGLY
13   ASNLYSPHETHRILETHRSERGLULEUMET
14   GLYLEUASPARGLYSLEUGLUASPTYRHIS
15   ALA

Samples:

sample_1: methane monooxygenase regulatory protein B, [U-13C 100%; U-15N 100%], 1 mM; phosphate 50 mM

sample_cond_1: pH: 6.5; temperature: 298 K; ionic strength: 0.05 M

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availablesample_cond_1
HN(CA)COsample_1not availablesample_cond_1
HNCAsample_1not availablesample_cond_1
HN(CO)CAsample_1not availablesample_cond_1
HNHAsample_1not availablesample_cond_1
HCCH-TOCSYsample_1not availablesample_cond_1
HC(CO)NHsample_1not availablesample_cond_1
H(CCO)NHsample_1not availablesample_cond_1
HNHBsample_1not availablesample_cond_1
NOESYsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz
  • Bruker DMX 600 MHz
  • Varian UnityPlus 400 MHz
  • Varian Unity 500 MHz

Related Database Links:

BMRB 17463
PDB
GB AAF04158 AAU92726
REF WP_010960484
SP P18797