BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4370

Title: 1H and 15N chemical shift assignments for HP-RNase

Deposition date: 1999-07-19 Original release date: 2000-12-14

Authors: El-Joubary, Abderraman; Bruix, Marta; Santoro, Jorge; Cafaro, Valeria; Scognamiglio, Roberta; Di Donato, Alberto; D'Alessio, Giuseppe; Kover, Katalin; Batta, Gyula; Szilagyi, Laszlo; Rico, Manuel

Citation: El-Joubary, Abderraman; Bruix, Marta; Santoro, Jorge; Cafaro, Valeria; Scognamiglio, Roberta; Di Donato, Alberto; D'Alessio, Giuseppe; Kover, Katalin; Batta, Gyula; Szilagyi, Laszlo; Rico, Manuel. "1H and 15N Sequential Assignment and Solution Secondary Structure of 15N Labelled Human Pancreatic Ribonuclease"  J. Biomol. NMR 15, 265-266 (1999).

Assembly members:
Human Pancreatic Ribonuclease, polymer, 128 residues, Formula weight is not available

Natural source:   Common Name: humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Human Pancreatic Ribonuclease: MKESRAKKFQRQHMDSDSSP SSSSTYCNQMMRRRNMTQGR CKPVNTFVHEPLVDVQNVCF QEKVTCKNGQGNCYKSNSSM HITDCRLTNGSRYPNCAYRT SPKERHIIVACEGSPYVPVH FDASVEDS

Data sets:
Data typeCount
15N chemical shifts143
1H chemical shifts795

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HP-RNase1

Entities:

Entity 1, HP-RNase 128 residues - Formula weight is not available

1   METLYSGLUSERARGALALYSLYSPHEGLN
2   ARGGLNHISMETASPSERASPSERSERPRO
3   SERSERSERSERTHRTYRCYSASNGLNMET
4   METARGARGARGASNMETTHRGLNGLYARG
5   CYSLYSPROVALASNTHRPHEVALHISGLU
6   PROLEUVALASPVALGLNASNVALCYSPHE
7   GLNGLULYSVALTHRCYSLYSASNGLYGLN
8   GLYASNCYSTYRLYSSERASNSERSERMET
9   HISILETHRASPCYSARGLEUTHRASNGLY
10   SERARGTYRPROASNCYSALATYRARGTHR
11   SERPROLYSGLUARGHISILEILEVALALA
12   CYSGLUGLYSERPROTYRVALPROVALHIS
13   PHEASPALASERVALGLUASPSER

Samples:

sample_1: Human Pancreatic Ribonuclease1 – 1.5 mM

sample_2: Human Pancreatic Ribonuclease, [U-95% 15N], 1 – 1.5 mM

sample_conditions_1: ionic strength: 0.6 .; pH: 4.2; temperature: 293 K

sample_conditions_2: ionic strength: 0.6 M; pH: 4.2; temperature: 298 K

sample_conditions_3: ionic strength: 0.6 M; pH: 4.2; temperature: 303 K

sample_conditions_4: ionic strength: 0.6 M; pH: 4.2; temperature: 308 K

sample_conditions_5: ionic strength: 0.6 M; pH*: 4.2; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
COSYnot availablenot availablenot available
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available
15N-HSQC-TOCSYnot availablenot availablenot available
15N-HSQC-NOESYnot availablenot availablenot available

Software:

XEASY v1.3.13 - semi-automated peak assignments

NMR spectrometers:

  • Bruker AMX 600 MHz

Related Database Links:

PDB
DBJ BAA05124 BAG35036
EMBL CAA44718 CAA55817 CAG29314 CAI47727
GB AAB35096 AAH05324 AAH22882 AAL87049 AAL87050
REF NP_001009108 NP_002924 NP_937875 NP_937877 NP_937878
SP P07998 Q8SQ11 Q8SQ13 Q8SQ14
TPE CDG31913 CDG31928 CDG31954

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts