BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4363

Title: NMR Structure of the Sea Urchin (Strongylocentrotus purpuratus) Metallothionein MTA

Authors: Riek, Roland; Precheur, Benedicte; Wang, Yunyuan; Mackay, Elaine; Wider, Gerhard; Guntert, Peter; Liu, Aizhuo; Kaegi, Jeremias; Wuthrich, Kurt

Citation: Riek, Roland; Precheur, Benedicte; Wang, Yunyuan; Mackay, Elaine; Wider, Gerhard; Guntert, Peter; Liu, Aizhuo; Kaegi, Jeremias; Wuthrich, Kurt. "NMR Structure of the Sea Urchin (Strongylocentrotus purpuratus) Metallothionein MTA"  J. Mol. Biol. 291, 417-428 (1999).

Assembly members:
Metallothionein MTA, polymer, 63 residues, Formula weight is not available
CD, non-polymer, 112.411 Da.

Natural source:   Common Name: purple urchin   Taxonomy ID: 7668   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Strongylocentrotus purpuratus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Metallothionein MTA: PDVKCVCCTEGKECACFGQD CCVTGECCKDGTCCGICTNA ACKCANGCKCGSGCSCTEGN CAC

Data typeCount
113Cd chemical shifts7
1H chemical shifts321

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Metallothionein MTA1
2CdII-12
3CdII-22
4CdII-32
5CdII-42
6CdII-52
7CdII-62
8CdII-72

Entities:

Entity 1, Metallothionein MTA 63 residues - Formula weight is not available

1   PROASPVALLYSCYSVALCYSCYSTHRGLU
2   GLYLYSGLUCYSALACYSPHEGLYGLNASP
3   CYSCYSVALTHRGLYGLUCYSCYSLYSASP
4   GLYTHRCYSCYSGLYILECYSTHRASNALA
5   ALACYSLYSCYSALAASNGLYCYSLYSCYS
6   GLYSERGLYCYSSERCYSTHRGLUGLYASN
7   CYSALACYS

Entity 2, CdII-1 - Cd - 112.411 Da.

1   CD

Samples:

sample_1: Metallothionein MTA, [U-113Cd], 4 mM

cond_1: pH: 7.0; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_1not availablecond_1

Software:

DYANA v1.1 -

NMR spectrometers:

  • Varian Unity+ 750 MHz

Related Database Links:

PDB
GB AAA30061
REF NP_999742