BMRB Entry 4359

Title:
NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly
Deposition date:
1999-06-14
Original release date:
1999-11-30
Authors:
Jabet, Carole; Gitti, Rossitza; Summers, Michael; Wolberger, Cynthia
Citation:

Citation: Jabet, Carole; Gitti, Rossitza; Summers, Michael; Wolberger, Cynthia. "NMR studies of the Pbx1 TALE Homeodomain Protein free in Solution and bound to DNA : Proposal for a Mechanism of HoxB1-Pbx1-DNA Complex Assembly"  J. Mol. Biol. 291, 521-530 (1999).

Assembly members:

Assembly members:
Pbx1, polymer, 19 residues, Formula weight is not available
Pbx1, polymer, 19 residues, Formula weight is not available
Pbx1, polymer, 87 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts74
13C chemical shifts71
15N chemical shifts74

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Pbx1 HD complex dna 11
2Pbx1 HD complex dna 22
3Pbx1 HD complex protein3

Entities:

Entity 1, Pbx1 HD complex dna 1 19 residues - Formula weight is not available

1   DCDTDCDTDADTDGDADTDT
2   DGDADTDCDGDGDCDTDG

Entity 2, Pbx1 HD complex dna 2 19 residues - Formula weight is not available

1   DCDADGDCDCDGDADTDCDA
2   DADTDCDADTDADGDADG

Entity 3, Pbx1 HD complex protein 87 residues - Formula weight is not available

1   ALAARGARGLYSARGARGASNPHEASNLYS
2   GLNALATHRGLUILELEUASNGLUTYRPHE
3   TYRSERHISLEUSERASNPROTYRPROSER
4   GLUGLUALALYSGLUGLULEUALALYSLYS
5   CYSGLYILETHRVALSERGLNVALSERASN
6   TRPPHEGLYASNLYSARGILEARGTYRLYS
7   LYSASNILEGLYLYSPHEGLNGLUGLUALA
8   ASNILETYRALAALALYSTHRALAVALTHR
9   ALATHRASNVALSERALAHIS

Samples:

sample_1: Pbx1 homeodomain, [U-90% 15N], 1 mM

sample_2: Pbx1 homeodomain, [U-95% 13C; U-90% 15N], 1 mM

sample_conditions_1: pH: 7 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available

Software:

NMRPipe - data processing

NMRView - peak assignments

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4357 4358
PDB
DBJ BAA96135 BAA96136 BAB83538 BAF84104 BAG10741
GB AAA21832 AAA36484 AAA36764 AAA60031 AAB71191
PIR B33061
REF NP_001094151 NP_001128334 NP_001179697 NP_001191890 NP_001191892
SP P40424 P41778
TPG DAA32038
AlphaFold P41778 P40424

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks