BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4340

Title: Complete 1H, 15N and 13C Assignment of a Recombinant Mouse Major Urinary Protein

Authors: Abbate, Francesco; Franzoni, Lorella; Loehr, Frank; Luecke, Christian; Ferrari, Elena; Sorbi, Robert; Rueterjans, Heinz; Spisni, Alberto

Citation: Abbate, Francesco; Franzoni, Lorella; Loehr, Frank; Luecke, Christian; Ferrari, Elena; Sorbi, Robert; Rueterjans, Heinz; Spisni, Alberto. "Complete 1H, 15N and 13C Assignment of a Recombinant Mouse Major Urinary Protein"  J. Biomol. NMR 15, 187-188 (1999).

Assembly members:
Mouse major urinary protein, polymer, 162 residues, 18707 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Pichia pastoris

Entity Sequences (FASTA):
Mouse major urinary protein: EEASSTGRNFNVEKINGEWH TIILASDKREKIEDNGNFRL FLEQIHVLEKSLVLKFHTVR DEECSELSMVADKTEKAGEY SVTYDGFNTFTIPKTDYDNF LMAHLINEKDGETFQLMGLY GREPDLSSDIKERFAQLCEE HGILRENIIDLSNANRCLQA RE

Data sets:
Data typeCount
1H chemical shifts1152
13C chemical shifts771
15N chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rMUP1

Entities:

Entity 1, rMUP 162 residues - 18707 Da.

1   GLUGLUALASERSERTHRGLYARGASNPHE
2   ASNVALGLULYSILEASNGLYGLUTRPHIS
3   THRILEILELEUALASERASPLYSARGGLU
4   LYSILEGLUASPASNGLYASNPHEARGLEU
5   PHELEUGLUGLNILEHISVALLEUGLULYS
6   SERLEUVALLEULYSPHEHISTHRVALARG
7   ASPGLUGLUCYSSERGLULEUSERMETVAL
8   ALAASPLYSTHRGLULYSALAGLYGLUTYR
9   SERVALTHRTYRASPGLYPHEASNTHRPHE
10   THRILEPROLYSTHRASPTYRASPASNPHE
11   LEUMETALAHISLEUILEASNGLULYSASP
12   GLYGLUTHRPHEGLNLEUMETGLYLEUTYR
13   GLYARGGLUPROASPLEUSERSERASPILE
14   LYSGLUARGPHEALAGLNLEUCYSGLUGLU
15   HISGLYILELEUARGGLUASNILEILEASP
16   LEUSERASNALAASNARGCYSLEUGLNALA
17   ARGGLU

Samples:

15N_sample: Mouse major urinary protein, [U-15N], 2.7 mM

13C_15N_sample: Mouse major urinary protein, [U-13C; U-15N], 1.7 mM

rMUP_exp_cond: pH: 7.2 na; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
(HCA)CO(CA)NHnot availablenot availablenot available
HCACOnot availablenot availablenot available
H(N)CA,COnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HBHA(CC)(CO)NHnot availablenot availablenot available
CC(CO)NH-TOCSYnot availablenot availablenot available
H(C)CH-TOCSYnot availablenot availablenot available
(H)CB(CGC)CH-TOCSYnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-13C NOESY-HSQCnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available

Software:

XWIN-NMR v1.3 - NMR data processing

AURELIA v2.5.9 - NMR data analysis

NMR spectrometers:

  • BRUKER DMX 600 MHz
  • BRUKER DMX 500 MHz

Related Database Links:

BMRB 17447
PDB
DBJ BAB27604 BAB28753 BAB29093
EMBL CAA26953 CAA27227 CAA27729 CAC34259 CAQ11104
GB AAA39764 AAA39765 AAA39767 AAA39768 AAB47130
REF NP_001039015 NP_001116119 NP_001128116 NP_001128147 NP_001128148
SP B5X0G2 P02762 P04938 P11588 P11589
TPG DAA06299 DAA06300 DAA06301 DAA06302 DAA06303