BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4310

Title: Calmodulin SEF2-1 Complex   PubMed: 11266605

Authors: Larsson, Goran; Schleucher, Jurgen; Onions, Jacquline; Hermann, Stefan; Grundstrom, Thomas; Wijmenga, Sybren

Citation: Larsson, Goran; Schleucher, Jurgen; Onions, Jacquline; Hermann, Stefan; Grundstrom, Thomas; Wijmenga, Sybren. "A Novel Target Recognition Revealed by Calmodulin in Complex with the Basic Helix-loop-helix Transcription Factor SEF2-1/E2-2"  Protein Sci. 10, 169-186 (2001).

Assembly members:
calmodulin, polymer, 148 residues, 16700 Da.
SL3 Enhancer Factor2-1, polymer, 21 residues, 2500 Da.
CA, non-polymer, 40.078 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK
SL3 Enhancer Factor2-1: KERRMANNARERLRVRGGCG W

Data sets:
Data typeCount
13C chemical shifts577
15N chemical shifts144
1H chemical shifts1139
coupling constants125

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin one1
2calmodulin two1
3SL3 Enhancer Factor2-1 one2
4SL3 Enhancer Factor2-1 two2
5CALCIUM ION, I3
6CALCIUM ION, II3
7CALCIUM ION, III3
8CALCIUM ION, IV3
9CALCIUM ION, V3
10CALCIUM ION, VI3
11CALCIUM ION, VII3
12CALCIUM ION, VIII3

Entities:

Entity 1, calmodulin one 148 residues - 16700 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 2, SL3 Enhancer Factor2-1 one 21 residues - 2500 Da.

1   LYSGLUARGARGMETALAASNASNALAARG
2   GLUARGLEUARGVALARGGLYGLYCYSGLY
3   TRP

Entity 3, CALCIUM ION, I - Ca - 40.078 Da.

1   CA

Samples:

sample_one: calmodulin, [U-13C; U-15N], 1.0 mM; SL3 Enhancer Factor2-1 1.0 mM; CALCIUM (II) ION 8.0 mM

sample_two: calmodulin, [U-15N], 1.0 mM; SL3 Enhancer Factor2-1 1.0 mM; CALCIUM (II) ION 8.0 mM

sample_conditions_one: pH: 6.0; temperature: 306 K

Experiments:

NameSampleSample stateSample conditions
F1, F2 (13C/15N) filtered 1H-1H NOESYsample_twonot availablesample_conditions_one
F1 (13C/15N) filtered 1H-1H TOCSYsample_twonot availablesample_conditions_one
1H-13C CT-HSQCsample_twonot availablesample_conditions_one
(HB)CB(CGCD)HDsample_twonot availablesample_conditions_one
CBCANHsample_twonot availablesample_conditions_one
CBCACO(N)Hsample_twonot availablesample_conditions_one
HNCOsample_twonot availablesample_conditions_one
HBHACO(N)Hsample_twonot availablesample_conditions_one
CTSL-HCANHsample_twonot availablesample_conditions_one
3D 1H-1H-15N NOESYsample_twonot availablesample_conditions_one
3D 1H-1H-13C NOESYsample_twonot availablesample_conditions_one
(H)C(C)(CO)NH TOCSYsample_twonot availablesample_conditions_one
H(CC)(CO)NH TOCSYsample_twonot availablesample_conditions_one

Software:

TRIPOS - data analysis

XWINNMR - processing

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker AMX-2 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4270 4284
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
SP O02367 O16305 O96081 P02594 P05932
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988