BMRB Entry 4302

Title:
Assignment of 1H, 13C and 15N Resonances of the a' Domain of Protein Disulfide Isomerase
Deposition date:
1999-01-28
Original release date:
1999-08-12
Authors:
Dijkstra, Klaas; Karvonen, Paeivi; Huovinen, Annamari; Koivunen, Peppi; Kivirikko, Kari; Darby, Nigel; van Straaten, Monique; Scheek, Ruud; Kemmink, Johan
Citation:

Citation: Dijkstra, Klaas; Karvonen, Paeivi; Huovinen, Annamari; Koivunen, Peppi; Kivirikko, Kari; Darby, Nigel; van Straaten, Monique; Scheek, Ruud; Kemmink, Johan. "Assignment of 1H, 13C and 15N Resonances of the a' Domain of Protein Disulfide Isomerase"  J. Biomol. NMR ., .-..

Assembly members:

Assembly members:
Protein Dilsulfide Isomerase a' domain, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET12a

Entity Sequences (FASTA):

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts519
15N chemical shifts114
1H chemical shifts778

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PDI a'1

Entities:

Entity 1, PDI a' 115 residues - Formula weight is not available

1   ASPLYSGLNPROVALLYSVALLEUVALGLY
2   LYSASNPHEGLUASPVALALAPHEASPGLU
3   LYSLYSASNVALPHEVALGLUPHETYRALA
4   PROTRPCYSGLYHISCYSLYSGLNLEUALA
5   PROILETRPASPLYSLEUGLYGLUTHRTYR
6   LYSASPHISGLUASNILEVALILEALALYS
7   METASPSERTHRALAASNGLUVALGLUALA
8   VALLYSVALHISSERPHEPROTHRLEULYS
9   PHEPHEPROALASERALAASPARGTHRVAL
10   ILEASPTYRASNGLYGLUARGTHRLEUASP
11   GLYPHELYSLYSPHELEUGLUSERGLYGLY
12   GLNASPGLYALAGLY

Samples:

sample_1: Protein Dilsulfide Isomerase a' domain, [U-13C], 1.0 – 2.0 mM

sample_2: Protein Dilsulfide Isomerase a' domain, [U-13C; U-15N], 1.0 – 2.0 mM

conditions_1: pH: 6.8 na; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

BMRB 11114
PDB
DBJ BAE39791 BAG53171 BAG54732
EMBL CAA30112
GB AAH14504 AIC63098 EAW89691 EAW89694 EAW89696
REF XP_008011468 XP_011850754 XP_011850814 XP_012306507

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks