BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4202

Title: Solution Structure of Reduced Monomeric Q133M2 Copper, Zinc Superoxide Dismutase(SOD). Why SOD is a Dimeric Enzyme?

Authors: Banci, Lucia; Benedetto, Marco; Bertini, Ivano; Del Conte, Rebecca; Piccioli, Mario; Viezzoli, Maria

Citation: Banci, Lucia; Benedetto, Marco; Bertini, Ivano; Del Conte, Rebecca; Piccioli, Mario; Viezzoli, Maria. "Solution Structure of Reduced Monomeric Q133M2 Copper, Zinc Superoxide Dismutase (SOD). Why SOD is a Dimeric Enzyme?"  Biochemistry 37, 11780-11791 (1998).

Assembly members:
Superoxide Dismutase, polymer, 153 residues, 16000 Da.
COPPER (II) ION, non-polymer, 63.546 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Superoxide Dismutase: ATKAVAVLKGDGPVQGIINF EQKESNGPVKVWGSIKGLTE GLHGFHVHEEEDNTAGCTSA GPHFNPLSRKHGGPKDEERH VGDLGNVTADKDGVADVSIE DSVISLSGDHSIIGRTLVVH EKADDLGKGGNEQSTKTGNA GSRLACGVIGIAQ

Data sets:
Data typeCount
13C chemical shifts579
15N chemical shifts157
1H chemical shifts959

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Q133M2 SOD1
2CU2
3Zn3

Entities:

Entity 1, Q133M2 SOD 153 residues - 16000 Da.

1   ALATHRLYSALAVALALAVALLEULYSGLY
2   ASPGLYPROVALGLNGLYILEILEASNPHE
3   GLUGLNLYSGLUSERASNGLYPROVALLYS
4   VALTRPGLYSERILELYSGLYLEUTHRGLU
5   GLYLEUHISGLYPHEHISVALHISGLUGLU
6   GLUASPASNTHRALAGLYCYSTHRSERALA
7   GLYPROHISPHEASNPROLEUSERARGLYS
8   HISGLYGLYPROLYSASPGLUGLUARGHIS
9   VALGLYASPLEUGLYASNVALTHRALAASP
10   LYSASPGLYVALALAASPVALSERILEGLU
11   ASPSERVALILESERLEUSERGLYASPHIS
12   SERILEILEGLYARGTHRLEUVALVALHIS
13   GLULYSALAASPASPLEUGLYLYSGLYGLY
14   ASNGLUGLNSERTHRLYSTHRGLYASNALA
15   GLYSERARGLEUALACYSGLYVALILEGLY
16   ILEALAGLN

Entity 2, CU - Cu - 63.546 Da.

1   CU

Entity 3, Zn - Zn - 65.409 Da.

1   ZN

Samples:

sample_one: Superoxide Dismutase, [U-13C; U-15N], 2 – 3 mM

sample_conditions_one: pH: 5.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 600 MHz
  • Bruker DRX 600 MHz
  • Varian Avance 800 MHz
  • Varian Avance 600 MHz

Related Database Links:

BMRB 15711 15712 15713 15714 18509 18708 18968 26570
PDB
GB AAA72747 AAA80237