BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4151

Title: 1H, 13C, and 15N Assignment and Secondary Structure of the High pH Form of Subunit c of the F1FO ATP Synthase

Authors: Rastogi, Vinit; Girvin, Mark

Citation: Rastogi, Vinit; Girvin, Mark. "1H, 13C, and 15N Assignment and Secondary Structure of the High pH Form of Subunit c of the F1FO ATP Synthase"  J. Biomol. NMR 13, 91-92 (1999).

Assembly members:
F1FO ATP Synthase Subunit c, polymer, 79 residues, Formula weight is not available

Natural source:   Common Name: Escherichia coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
F1FO ATP Synthase Subunit c: XENLNMDLLYMAAAVMMGLA AIGAAIGIGILGGKFLEGAA RQPDLIPLLRTQFFIVMGLV DAIPMIAVGLGLYVMFAVA

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts80
1H chemical shifts503

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1F1FO-ATP-c1

Entities:

Entity 1, F1FO-ATP-c 79 residues - Formula weight is not available

1   FMEGLUASNLEUASNMETASPLEULEUTYR
2   METALAALAALAVALMETMETGLYLEUALA
3   ALAILEGLYALAALAILEGLYILEGLYILE
4   LEUGLYGLYLYSPHELEUGLUGLYALAALA
5   ARGGLNPROASPLEUILEPROLEULEUARG
6   THRGLNPHEPHEILEVALMETGLYLEUVAL
7   ASPALAILEPROMETILEALAVALGLYLEU
8   GLYLEUTYRVALMETPHEALAVALALA

Samples:

sample_one: F1FO ATP Synthase Subunit c, [U98%-13C; U98%-15N], 1.5 – 2.0 mM

sample_conditions_one: pH: 8.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_onenot availablesample_conditions_one
CT 1H-13C HSQCsample_onenot availablesample_conditions_one
HNCOsample_onenot availablesample_conditions_one
1H-15N TOCSY-HSQCsample_onenot availablesample_conditions_one
H(CCO)NHsample_onenot availablesample_conditions_one
C(CO)NHsample_onenot availablesample_conditions_one
HNCACBsample_onenot availablesample_conditions_one
HCCH-COSYsample_onenot availablesample_conditions_one
HCCH-TOCSYsample_onenot availablesample_conditions_one
1H-15N HMQC-NOESY-HSQCsample_onenot availablesample_conditions_one
1H-15N NOESY-HSQCsample_onenot availablesample_conditions_one
HNHAsample_onenot availablesample_conditions_one

Software:

NMRPipe v1.6 - Spectral data processing

NMR spectrometers:

  • Bruker DRX 600 MHz

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