BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4138

Title: Proton Resonance Assignments of RC-RNase

Deposition date: 1998-05-06 Original release date: 1998-06-15

Authors: Chen, Chinpan; Hom, Kellie; Huang, Rong-Fong; Chou, Ping-Jung; Laio, You-Di; Huang, Tai-huang

Citation: Chen, Chinpan; Hom, Kellie; Huang, Rong-Fong; Chou, Ping-Jung; Laio, You-Di; Huang, Tai-huang. "The Secondary Structure of a Pyrimidine-Guanine Sequence-Specific Ribonuclease Possessing Cytotoxic Activity from the Oocytes of Rana catesbeiana"  J. Biomol NMR 8, 331-344 (1996).

Assembly members:
RC-RNase, polymer, 111 residues, 12440 Da.

Natural source:   Common Name: bull-frog   Taxonomy ID: 8400   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rana Catesbeiana

Experimental source:   Production method: purified from the natural source

Entity Sequences (FASTA):
RC-RNase: XNWATFQQKHIINTPIINCN TIMDNNIYIVGGQCKRVNTF IISSATTVKAICTGVINMNV LSTTRFQLNTCTRTSITPRP CPYSSRTETNYICVKCENQY PVHFAGIGRCP

Data typeCount
1H chemical shifts768

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RC-RNase1

Entities:

Entity 1, RC-RNase 111 residues - 12440 Da.

1   PCAASNTRPALATHRPHEGLNGLNLYSHIS
2   ILEILEASNTHRPROILEILEASNCYSASN
3   THRILEMETASPASNASNILETYRILEVAL
4   GLYGLYGLNCYSLYSARGVALASNTHRPHE
5   ILEILESERSERALATHRTHRVALLYSALA
6   ILECYSTHRGLYVALILEASNMETASNVAL
7   LEUSERTHRTHRARGPHEGLNLEUASNTHR
8   CYSTHRARGTHRSERILETHRPROARGPRO
9   CYSPROTYRSERSERARGTHRGLUTHRASN
10   TYRILECYSVALLYSCYSGLUASNGLNTYR
11   PROVALHISPHEALAGLYILEGLYARGCYS
12   PRO

Samples:

sample_one: RC-RNase2 – 3 mM; H2O 90%; D2O 10%; phosphate buffer 50 mM

sample_conditions_one: pH: 3.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
GB AAD10702
SP P11916