BMRB Entry 4136

Title:
Chemical Shift Assignments, 3JHNHA Coupling Constants and Secondary Structure of E.coli Multidrug Resistance Protein (EmrE)
Deposition date:
1998-04-23
Original release date:
1998-07-02
Authors:
Schwaiger, Manfred; Lebendiker, Mario; Yerushalmi, Hagit; Coles, Murray; Groeger, Adriane; Schwarz, Christian; Schuldiner, Shimon; Kessler, Horst
Citation:

Citation: Schwaiger, Manfred; Lebendiker, Mario; Yerushalmi, Hagit; Coles, Murray; Groeger, Adriane; Schwarz, Christian; Schuldiner, Shimon; Kessler, Horst. "NMR-Investigations of the Multidrug Transporter EmrE, an Integral Membrane Protein"  Eur. J. Biochem. ., 610-619 (1998).

Assembly members:

Assembly members:
E. coli Multidrug resistance protein E, polymer, 110 residues, 12000 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK56

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts283
15N chemical shifts113
1H chemical shifts739
coupling constants73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1EmrE1

Entities:

Entity 1, EmrE 110 residues - 12000 Da.

1   METASNPROTYRILETYRLEUGLYGLYALA
2   ILELEUALAGLUVALILEGLYTHRTHRLEU
3   METLYSPHESERGLUGLYPHETHRARGLEU
4   TRPPROSERVALGLYTHRILEILECYSTYR
5   CYSALASERPHETRPLEULEUALAGLNTHR
6   LEUALATYRILEPROTHRGLYILEALATYR
7   ALAILETRPSERGLYVALGLYILEVALLEU
8   ILESERLEULEUSERTRPGLYPHEPHEGLY
9   GLNARGLEUASPLEUPROALAILEILEGLY
10   METMETLEUILECYSALAGLYVALLEUILE
11   ILEASNLEULEUSERARGSERTHRPROHIS

Samples:

sample_one: EmrE, [U-15N], 2.0 mM; ammonium acetate 200 mM; D-chloroform 46.2%; D3-methanol 46.2%; H2O 7.6%

sample_two: EmrE, [U-15N; U-13C], 1.5 mM; ammonium acetate 200 mM; D-chloroform 46.2%; D3-methanol 46.2%; H2O 7.6%

sample_three: EmrE, [U-15N; U-13C; U-75% 2H], 1.0 mM; ammonium acetate 200 mM; D-chloroform 46.2%; D3-methanol 46.2%; H2O 7.6%

sample_conditions_one: pH: 6.2; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • Bruker DMX750 750 MHz
  • Bruker DMX600 600 MHz

Related Database Links:

PDB
DBJ BAB35037 BAE76318 BAG76102 BAG77691 BAI36380
EMBL CAA77936 CAP75647 CAP76416 CAQ31015 CAR03289
GB AAA24190 AAB40740 AAC73644 AAG55967 AAN43529
PRF 1803250A
REF NP_309641 NP_415075 NP_707822 WP_001070433 WP_001070437
SP P23895
AlphaFold P23895

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks