BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4135

Title: Assignments of a RNA Duplex Including the C-U Mismatch: Unusual Chemical Shifts of a Cytidine Amino Group in the C-U Pair.

Authors: Tanaka, Yoshiyuki; Kojima, Chojiro; Yamazaki, Toshio; Kodama, T.; Yasuno, Kazuhiro; Miyashita, Shin; Ono, Akira(Mei); Ono, Akira(Sho); Kainosho, Masatsune; Kyogoku, Yoshimasa

Citation: Tanaka, Yoshiyuki; Kojima, Chojiro; Yamazaki, Toshio; Kodama, T.; Yasuno, Kazuhiro; Miyashita, Shin; Ono, Akira(Mei); Ono, Akira(Sho); Kainosho, Masatsune; Kyogoku, Yoshimasa. "Solution Structure of an RNA Duplex Including a C-U Base Pair"  Biochemistry 39, 7074-7080 (2000).

Assembly members:
RNA_duplex_strand_A, polymer, 9 residues, 3000 Da.
RNA_duplex_strand_B, polymer, 9 residues, 3000 Da.

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
RNA_duplex_strand_A: CGACUCAGG
RNA_duplex_strand_B: CCUGCGUCG

Data typeCount
15N chemical shifts1
31P chemical shifts16

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1strand_A1
2strand_B2

Entities:

Entity 1, strand_A 9 residues - 3000 Da.

1   CGACUCAGG

Entity 2, strand_B 9 residues - 3000 Da.

1   CCUGCGUCG

Samples:

sample_one: RNA_duplex_strand_A, [15N4]-C14, 2.0 mM; NaHPO4 20 mM; NaCl 50 mM

sample_two: RNA_duplex_strand_B, [15N4]-C14, 2.0 mM; NaHPO4 20 mM; NaCl 50 mM

sample_conditions_one: pH: 7.0; temperature: 303 K

sample_conditions_two: pH: 7.0; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

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