BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4086

Title: Tumor Suppressor p16ink4a: Structural Characterization of Wild-Type and Mutant Proteins by NMR and Circular Dichroism   PubMed: 8755727

Authors: Tevelev, Anton; Byeon, In-Ja; Selby, Thomas; Ericson, Karen; Kim, Hee-Jung; Kraynov, Vadim; Tsai, Ming-Daw

Citation: Tevelev, Anton; Byeon, In-Ja; Selby, Thomas; Ericson, Karen; Kim, Hee-Jung; Kraynov, Vadim; Tsai, Ming-Daw. "Tumor Suppressor p16ink4a: Structural Characterization of Wild-Type and Mutant Proteins by NMR and Circular Dichroism"  Biochemistry 35, 9475-9487 (1996).

Assembly members:
p16(INK4)/delta_1-8, polymer, 148 residues, 16569 Da.

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
p16(INK4)/delta_1-8: MEPSADWLATAAARGRVEEV RALLEAGALPNAPNSYGRRP IQVMMMGSARVAELLLLHGA EPNCADPATLTRPVHDAARE GFLDTLVVLHRAGARLDVRD AWGRLPVDLAEELGHRDVAR YLRAAAGGTRGSNHARIDAA EGPSDIPD

Data sets:
Data typeCount
13C chemical shifts330
15N chemical shifts123
1H chemical shifts684

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1p16(INK4)/delta_1-81

Entities:

Entity 1, p16(INK4)/delta_1-8 148 residues - 16569 Da.

1   METGLUPROSERALAASPTRPLEUALATHR
2   ALAALAALAARGGLYARGVALGLUGLUVAL
3   ARGALALEULEUGLUALAGLYALALEUPRO
4   ASNALAPROASNSERTYRGLYARGARGPRO
5   ILEGLNVALMETMETMETGLYSERALAARG
6   VALALAGLULEULEULEULEUHISGLYALA
7   GLUPROASNCYSALAASPPROALATHRLEU
8   THRARGPROVALHISASPALAALAARGGLU
9   GLYPHELEUASPTHRLEUVALVALLEUHIS
10   ARGALAGLYALAARGLEUASPVALARGASP
11   ALATRPGLYARGLEUPROVALASPLEUALA
12   GLUGLULEUGLYHISARGASPVALALAARG
13   TYRLEUARGALAALAALAGLYGLYTHRARG
14   GLYSERASNHISALAARGILEASPALAALA
15   GLUGLYPROSERASPILEPROASP

Samples:

sample_one: p16(INK4)/delta_1-8 0.2 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; D2O 10%; H2O 90%

sample_two: p16(INK4)/delta_1-8 0.2 mM; HEPES 4 mM; DTT 1 mM; EDTA 5 uM; D2O 100%

sample_conditions: pH: 7.0; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

BMRB 4526
PDB
DBJ BAA33541 BAB91133
EMBL CCQ43815
GB AAA92554 AAB32713 AAB60645 AAD14048 AAD14050
PRF 2002364A
REF NP_000068 NP_001139762 NP_001182061 XP_003830733 XP_005251400
SP P42771