BMRB Entry 4078

Title:
1H, 15N and 13C Resonance Assignments and Secondary Structure of Group II Phospholipase A2 from Agkistrodon piscivorus piscivorus: Presence of an Amino-Terminal Helix in Solution
Deposition date:
1997-12-07
Original release date:
2001-02-16
Authors:
Jerala, Roman; Almeida, Paulo; Ye, Qiang; Biltonen, Rodney; Rule, Gordon
Citation:

Citation: Jerala, Roman; Almeida, Paulo; Ye, Qiang; Biltonen, Rodney; Rule, Gordon. "1H, 15N and 13C Resonance Assignments and Secondary Structure of Group II Phospholipase A2 from Agkistrodon piscivorus piscivorus: Presence of an Amino-Terminal Helix in Solution"  J. Biomol. NMR 7, 107-120 (1996).

Assembly members:

Assembly members:
phospholipase A2, polymer, 123 residues, 14000 Da.

Natural source:

Natural source:   Common Name: eastern cottonmouth   Taxonomy ID: 8715   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Agkistrodon piscivorus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Eschericia coli

Data sets:
Data typeCount
1H chemical shifts735
13C chemical shifts462
15N chemical shifts129

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1App-D49 PLA21

Entities:

Entity 1, App-D49 PLA2 123 residues - 14000 Da.

1   SERLEUPHEGLNPHEGLULYSLEUILELYS
2   LYSMETTHRGLYLYSSERGLYMETLEUTRP
3   TYRSERALATYRGLYCYSTYRCYSGLYTRP
4   GLYGLYGLNGLYARGPROLYSASPALATHR
5   ASPARGCYSCYSPHEVALHISASPCYSCYS
6   TYRGLYLYSVALTHRGLYCYSASNPROLYS
7   METASPILETYRTHRTYRSERVALGLUASN
8   GLYASNILEVALCYSGLYGLYTHRASNPRO
9   CYSLYSLYSGLNILECYSGLUCYSASPARG
10   ALAALAALAILECYSPHEARGASPASNLEU
11   LYSTHRTYRASPSERLYSTHRTYRTRPLYS
12   TYRPROLYSLYSASNCYSLYSGLUGLUSER
13   GLUPROCYS

Samples:

sample_one: phospholipase A2, [U-15N], 0.2 mM; sodium phosphate 20 mM; KCL 100 mM; D2O 5%; H2O 95%

sample_two: phospholipase A2, [U-13C; U-15N], 0.2 mM; sodium phosphate 20 mM; KCL 100 mM; D2O 5%; H2O 95%

sample_conditions: pH: 4.3; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions

Software:

No software information available

NMR spectrometers:

  • Varian UnityPlus 500 MHz

Related Database Links:

PDB
GB AAB25792 AAB26655
SP P51972
AlphaFold P51972

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks