BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4051

Title: 1H, 13C, and 15N Chemical Shift Assignments for Oxidized Clostridium pasteurianum Rubredoxin

Authors: Prantner, Andrew; Volkman, Brian; Wilkens, Steven; Xia, Bin; Markley, John

Citation: Volkman, Brian; Prantner, Andrew; Wilkens, Steven; Xia, Bin; Markley, John. "Assignment of 1H, 13C, 15N Signals of Oxidized Clostridium Pasteurianum Rubredoxin"  J. Biomol. NMR 10, 409-410 (1997).

Assembly members:
rubredoxin peptide, polymer, 54 residues, 6040 Da.
FE, non-polymer, 55.845 Da.

Natural source:   Common Name: C. pasteurianum   Taxonomy ID: 1501   Superkingdom: Eubacteria   Kingdom: Monera   Genus/species: Clostridium pasteurianum

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
rubredoxin peptide: MKKYTCTVCGYIYNPEDGDP DNGVNPGTDFKDIPDDWVCP LCGVGKDQFEEVEE

Data sets:
Data typeCount
13C chemical shifts118
15N chemical shifts31
1H chemical shifts179

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1rubredoxin1
2Fe(III)2

Entities:

Entity 1, rubredoxin 54 residues - 6040 Da.

1   METLYSLYSTYRTHRCYSTHRVALCYSGLY
2   TYRILETYRASNPROGLUASPGLYASPPRO
3   ASPASNGLYVALASNPROGLYTHRASPPHE
4   LYSASPILEPROASPASPTRPVALCYSPRO
5   LEUCYSGLYVALGLYLYSASPGLNPHEGLU
6   GLUVALGLUGLU

Entity 2, Fe(III) - Fe - 55.845 Da.

1   FE

Samples:

sample_one: rubredoxin peptide4 – 6 mM; Phosphate buffer 50 mM

sample_conditions_one: pH: 6.0 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown . MHz

Related Database Links:

BMRB 4050 4066 4137 4182 4319 4320
PDB
GB AAA23279 AJA49845 AJA53833 ELP57804 KER11884
REF WP_003447684
SP P00268