BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 4030

Title: Refinement of the Solution Structure of Calcium-Free, Myristoylated Recoverin

Authors: Tanaka, Toshiyuki; Ames, James; Kainosho, Masatsune; Stryer, Lubert; Ikura, Mitsuhiko

Citation: Tanaka, Toshiyuki; Ames, James; Kainosho, Masatsune; Stryer, Lubert; Ikura, Mitsuhiko. "Differential isotype labeling strategy for determining the structure of myristoylated recoverin by NMR spectroscopy"  J. Biomol. NMR 11, 135-152 (1998).

Assembly members:
recoverin, polymer, 202 residues, Formula weight is not available

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
recoverin: XGNSKSGALSKEILEELQLN TKFTEEELSSWYQSFLKECP SGRITRQEFQTIYSKFFPEA DPKAYAQHVFRSFDANSDGT LDFKEYVIALHMTSAGKTNQ KLEWAFSLYDVDGNGTISKN EVLEIVTAIFKMISPEDTKH LPEDENTPEKRAEKIWGFFG KKDDDKLTEKEFIEGTLANK EILRLIQFEPQKVKEKLKEK KL

Data sets:
  • assigned_chemical_shifts
Data typeCount
13C chemical shifts827
15N chemical shifts207
1H chemical shifts1241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-recoverin1

Entities:

Entity 1, apo-recoverin 202 residues - Formula weight is not available

1   MYRGLYASNSERLYSSERGLYALALEUSER
2   LYSGLUILELEUGLUGLULEUGLNLEUASN
3   THRLYSPHETHRGLUGLUGLULEUSERSER
4   TRPTYRGLNSERPHELEULYSGLUCYSPRO
5   SERGLYARGILETHRARGGLNGLUPHEGLN
6   THRILETYRSERLYSPHEPHEPROGLUALA
7   ASPPROLYSALATYRALAGLNHISVALPHE
8   ARGSERPHEASPALAASNSERASPGLYTHR
9   LEUASPPHELYSGLUTYRVALILEALALEU
10   HISMETTHRSERALAGLYLYSTHRASNGLN
11   LYSLEUGLUTRPALAPHESERLEUTYRASP
12   VALASPGLYASNGLYTHRILESERLYSASN
13   GLUVALLEUGLUILEVALTHRALAILEPHE
14   LYSMETILESERPROGLUASPTHRLYSHIS
15   LEUPROGLUASPGLUASNTHRPROGLULYS
16   ARGALAGLULYSILETRPGLYPHEPHEGLY
17   LYSLYSASPASPASPLYSLEUTHRGLULYS
18   GLUPHEILEGLUGLYTHRLEUALAASNLYS
19   GLUILELEUARGLEUILEGLNPHEGLUPRO
20   GLNLYSVALLYSGLULYSLEULYSGLULYS
21   LYSLEU

Samples:

sample_one: recoverin, [U-95% 15N; U-95% 13C], 1.0 – 1.5 mM; d12-EDTA 1 mM; d10-DTT 10 mM; KCl 100 mM; H2O 95%; D2O 5%

sample_conditions_one: pH: 6.9; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

NMRPipe v1.0 - FID transformations

PIPP - peak picking

X-PLOR v2.1 - structure determination

NMRDraw - FID transformations

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB 1IKU 1JSA 1LA3 1OMR 1OMV 1REC 2HET 2I94 4M2O 4M2P 4M2Q 4MLW
EMBL CAA44928
GB AAB59256 ELR47741
PRF 1802271A
REF NP_776590 XP_004012748 XP_005693626 XP_005907603 XP_005959860
SP P21457
TPG DAA18803