BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36299

Title: Solution structure of SPA-2 SHD

Deposition date: 2019-11-12 Original release date: 2020-05-05

Authors: Fan, J.; Wong, J.; Zheng, P.; Yang, D.; Jedd, G.

Citation: Fan, J.; Wong, J.; Zheng, P.; Yang, D.; Jedd, G.. "Spitzenkorper assembly mechanisms reveal conserved features of fungal and metazoan polarity scaffolds"  . ., .-..

Assembly members:
entity_1, polymer, 155 residues, 17781.943 Da.

Natural source:   Common Name: Neurospora crassa   Taxonomy ID: 5141   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Neurospora crassa

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MGSSHHHHHHSSGLVPRGSH MGENQELVLSDHYISLKRYL SATSRDGNPKPPPNKARDKL QRLTEVQFLELSTDVYDELK RREQVARRGPNAPPETAPPD FLLPQDNFHPKRNQARQKLS SLGPPRFRDLATDVFCELER RYPNFANLEMPLNAS

Data sets:
Data typeCount
13C chemical shifts458
15N chemical shifts134
1H chemical shifts779

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 155 residues - 17781.943 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METGLYGLUASNGLNGLULEUVALLEUSER
4   ASPHISTYRILESERLEULYSARGTYRLEU
5   SERALATHRSERARGASPGLYASNPROLYS
6   PROPROPROASNLYSALAARGASPLYSLEU
7   GLNARGLEUTHRGLUVALGLNPHELEUGLU
8   LEUSERTHRASPVALTYRASPGLULEULYS
9   ARGARGGLUGLNVALALAARGARGGLYPRO
10   ASNALAPROPROGLUTHRALAPROPROASP
11   PHELEULEUPROGLNASPASNPHEHISPRO
12   LYSARGASNGLNALAARGGLNLYSLEUSER
13   SERLEUGLYPROPROARGPHEARGASPLEU
14   ALATHRASPVALPHECYSGLULEUGLUARG
15   ARGTYRPROASNPHEALAASNLEUGLUMET
16   PROLEUASNALASER

Samples:

sample_1: SHD, [U-13C; U-15N], 2 ± 0.1 mM; D2O, [U-2H], 10%; H2O 90%; disodium hydrogen phosphate 10 ± 0.1 mM; sodium dihydrogen phospahte 10 ± 0.1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
4D NOESYsample_1isotropicsample_conditions_1

Software:

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky, Goddard - data analysis

TopSpin, Bruker Biospin - collection

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker AVANCE 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts