BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36177

Title: Structure of omega conotoxin Bu8

Authors: Liu, X.; Jiang, L.

Citation: Liu, X.; Jiang, L.. "Solution structure of omega conotoxin bu8"  . ., .-..

Assembly members:
Omega-conotoxin, polymer, 26 residues, 2767.225 Da.

Natural source:   Common Name: Bubble cone   Taxonomy ID: 89438   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus bullatus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Omega-conotoxin: CKRKGSSCRRTSYDCCTGSC RNGKCX

Data sets:
Data typeCount
1H chemical shifts135

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 26 residues - 2767.225 Da.

1   CYSLYSARGLYSGLYSERSERCYSARGARG
2   THRSERTYRASPCYSCYSTHRGLYSERCYS
3   ARGASNGLYLYSCYSNH2

Samples:

sample_1: Omega-conotoxin mM; sodium phosphate 50 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 250 mM; pH: 3.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMR spectrometers:

  • Bruker AvanceIII 850 MHz

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