BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36174

Title: SOLUTION NMR STRUCTURE OF A 14-MER DOUBLE STRANDED DNA DUPLEX CGCGAAATTTCGCG

Authors: Ganguly, S.; Basu, G.

Citation: Ganguly, S.; Basu, G.. "SOLUTION NMR STRUCTURE OF A 14-MER DOUBLE STRANDED DNA DUPLEX"  . ., .-..

Assembly members:
entity_1, polymer, 14 residues, 4280.792 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: CGCGAAATTTCGCG

Data sets:
Data typeCount
1H chemical shifts258

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_A1
2entity_B1

Entities:

Entity 1, entity_A 14 residues - 4280.792 Da.

1   DCDGDCDGDADADADTDTDT
2   DCDGDCDG

Samples:

sample_1: DNA (5'-D(*CP*GP*CP*GP*AP*AP*AP*TP*TP*TP*CP*GP*CP*G)-3') 300 uM; H2O 90%; D2O, [U-2H], 10%

sample_2: DNA (5'-D(*CP*GP*CP*GP*AP*AP*AP*TP*TP*TP*CP*GP*CP*G)-3') 300 uM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 10 mM; pH: 7.0 .; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 10 mM; pH: 7.0 .; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2

Software:

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN v3.0, Bruker Biospin - data analysis

NMR spectrometers:

  • Bruker AvanceIII 700 MHz

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