BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 36161

Title: Solution structure of the SBDbeta domain of yeast Ssa1   PubMed: 30228181

Deposition date: 2018-01-31 Original release date: 2018-10-12

Authors: Gong, W.; Hu, W.; Wu, H.; Perrett, S.

Citation: Gong, W.; Hu, W.; Xu, L.; Wu, H.; Wu, S.; Zhang, H.; Wang, J.; Jones, G.; Perrett, S.. "The C-terminal GGAP motif of Hsp70 mediates substrate recognition and stress response in yeast"  J. Biol. Chem. 293, 17663-17675 (2018).

Assembly members:
entity_1, polymer, 173 residues, 18774.189 Da.

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 559292   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces Saccharomyces cerevisiae

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: SSKTQDLLLLDVAPLSLGIE TAGGVMTKLIPRNSTIPTKK SEIFSTYADNQPGVLIQVFE GERAKTKDNNLLGKFELSGI PPAPRGVPQIEVTFDVDSNG ILNVSAVEKGTGKSNKITIT NDKGRLSKEDIEKMVAEAEK FKEEDEKESQRIASKNQLES IAYSLKNTISEAG

Data sets:
Data typeCount
13C chemical shifts732
15N chemical shifts176
1H chemical shifts1130

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 173 residues - 18774.189 Da.

1   SERSERLYSTHRGLNASPLEULEULEULEU
2   ASPVALALAPROLEUSERLEUGLYILEGLU
3   THRALAGLYGLYVALMETTHRLYSLEUILE
4   PROARGASNSERTHRILEPROTHRLYSLYS
5   SERGLUILEPHESERTHRTYRALAASPASN
6   GLNPROGLYVALLEUILEGLNVALPHEGLU
7   GLYGLUARGALALYSTHRLYSASPASNASN
8   LEULEUGLYLYSPHEGLULEUSERGLYILE
9   PROPROALAPROARGGLYVALPROGLNILE
10   GLUVALTHRPHEASPVALASPSERASNGLY
11   ILELEUASNVALSERALAVALGLULYSGLY
12   THRGLYLYSSERASNLYSILETHRILETHR
13   ASNASPLYSGLYARGLEUSERLYSGLUASP
14   ILEGLULYSMETVALALAGLUALAGLULYS
15   PHELYSGLUGLUASPGLULYSGLUSERGLN
16   ARGILEALASERLYSASNGLNLEUGLUSER
17   ILEALATYRSERLEULYSASNTHRILESER
18   GLUALAGLY

Samples:

sample_1: Ssa1, [U-13C; U-15N], 1 mM; H2O 90%; D2O, [U-99% 2H], 10%

sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D CCH-TOCSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D 15N-edited NOESYsample_1isotropicsample_conditions_1
3D 13C-edited NOESYsample_1isotropicsample_conditions_1
3D 13C-edited aromatic NOESYsample_1isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

  • Varian DD2 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts