BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36158

Title: Nukacin ISK-1 in inactive state   PubMed: 30272026

Authors: Kohda, D.; Fujinami, D.

Citation: Fujinami, Daisuke; Mahin, Abdullah-Al; Elsayed, Khaled M; Islam, Mohammad R; Nagao, Jun-Ichi; Roy, Urmi; Momin, Sabrina; Zendo, Takeshi; Kohda, Daisuke; Sonomoto, Kenji. "The lantibiotic nukacin ISK-1 exists in an equilibrium between active and inactive lipid-II binding states."  Commun. Biol. 1, 150-150 (2018).

Assembly members:
Lantibiotic nukacin, polymer, 27 residues, 2970.535 Da.

Natural source:   Common Name: Staphylococcus warneri   Taxonomy ID: 1292   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus warneri

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Lantibiotic nukacin: KKKSGVIPXVXHDCHMNXFQ FVFXCCS

Data sets:
Data typeCount
1H chemical shifts171

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 27 residues - 2970.535 Da.

1   LYSLYSLYSSERGLYVALILEPRODBBVAL
2   DALHISASPCYSHISMETASNDALPHEGLN
3   PHEVALPHEDBUCYSCYSSER

Samples:

sample_1: nukacin ISK-1 1.1 mM; sodium phosphate 10 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0 mM; pH: 6.0; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert P. - refinement

NMR spectrometers:

  • Bruker Avance 700 MHz

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