BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36084

Title: Solution structure of C-terminal domain of TRADD

Deposition date: 2017-05-15 Original release date: 2017-06-26

Authors: Lin, Z.; Zhang, N.

Citation: Lin, Z.; Zhang, N.. "Solution structure of the carboxyl-terminal domain of TRADD reveals a unique fold in the death domain superfamily"  . ., .-..

Assembly members:
entity_1, polymer, 127 residues, 14429.224 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MHHHHHHSSGRGSAQTFLFQ GQPVVNRPLSLKDQQTFARS VGLKWRKVGRSLQRGCRALR DPALDSLAYEYEREGLYEQA FQLLRRFVQAEGRRATLQRL VEALEENELTSLAEDLLGLT DPNGGLA

Data sets:
Data typeCount
13C chemical shifts384
15N chemical shifts126
1H chemical shifts810

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 127 residues - 14429.224 Da.

1   METHISHISHISHISHISHISSERSERGLY
2   ARGGLYSERALAGLNTHRPHELEUPHEGLN
3   GLYGLNPROVALVALASNARGPROLEUSER
4   LEULYSASPGLNGLNTHRPHEALAARGSER
5   VALGLYLEULYSTRPARGLYSVALGLYARG
6   SERLEUGLNARGGLYCYSARGALALEUARG
7   ASPPROALALEUASPSERLEUALATYRGLU
8   TYRGLUARGGLUGLYLEUTYRGLUGLNALA
9   PHEGLNLEULEUARGARGPHEVALGLNALA
10   GLUGLYARGARGALATHRLEUGLNARGLEU
11   VALGLUALALEUGLUGLUASNGLULEUTHR
12   SERLEUALAGLUASPLEULEUGLYLEUTHR
13   ASPPROASNGLYGLYLEUALA

Samples:

sample_1: TRADD DD, [U-99% 13C; U-99% 15N; U-98% 2H], 0.6 mM; D10-DTT 10 mM; H2O 95%; D2O, [U-2H], 5%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 301 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
4D 13C, 15N-edited NOESYsample_1isotropicsample_conditions_1

Software:

AMBER, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

NMRView, Johnson, One Moon Scientific - data analysis

NMRpipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Bruker Avance 800 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts