BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 36037

Title: Solution structure of a histone binding domain

Deposition date: 2016-11-29 Original release date: 2017-05-15

Authors: Mi, J.

Citation: Mi, J.. "Solution Structure Of A Histone Binding Domain"  . ., .-..

Assembly members:
entity_1, polymer, 124 residues, 13878.720 Da.

Natural source:   Common Name: Trypanosoma brucei   Taxonomy ID: 185431   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Trypanosoma brucei

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: MSQNRQLLYPREEMVSLVRS LDRPQENGLFSQDVLLQYPE LAESYTKVCPNRCDLATAAD RAAKGAYGYDVQLTTLKEDI RLMVNNCILFNGAEGAYADA ARTFEKFAMGKIDAYISQKV GGLE

Data sets:
Data typeCount
13C chemical shifts315
15N chemical shifts133
1H chemical shifts616

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 124 residues - 13878.720 Da.

1   METSERGLNASNARGGLNLEULEUTYRPRO
2   ARGGLUGLUMETVALSERLEUVALARGSER
3   LEUASPARGPROGLNGLUASNGLYLEUPHE
4   SERGLNASPVALLEULEUGLNTYRPROGLU
5   LEUALAGLUSERTYRTHRLYSVALCYSPRO
6   ASNARGCYSASPLEUALATHRALAALAASP
7   ARGALAALALYSGLYALATYRGLYTYRASP
8   VALGLNLEUTHRTHRLEULYSGLUASPILE
9   ARGLEUMETVALASNASNCYSILELEUPHE
10   ASNGLYALAGLUGLYALATYRALAASPALA
11   ALAARGTHRPHEGLULYSPHEALAMETGLY
12   LYSILEASPALATYRILESERGLNLYSVAL
13   GLYGLYLEUGLU

Samples:

sample_1: entity_1, [U-13C, U-15N], mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 0.8 mM; pH: 6.8; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

CYANA, GUNTERT, MUMENTHALER AND WUTHRICH - refinement

NMR spectrometers:

  • BRUKER DMX 600 MHz

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts