BMRB Entry 34784

Name: Solution structure of the RNA helix formed by the 5'-end of U1 snRNA and an A-1 bulged 5'-splice site in complex with SMN-CY
Published: 2024-02-13

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PDB ID: 8cf2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34784
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the RNA helix formed by the 5'-end of U1 snRNA and an A-1 bulged 5'-splice site in complex with SMN-CY

Deposition date:

2023-02-02

Original release date:

2024-02-13

Authors:

Malard, F.; Marquevielle, J.; Campagne, S.

Citation:

Citation: Malard, F.; Marquevielle, J.; Campagne, S.. "The diversity of splicing modifiers acting on A-1 bulged 5'-splice sites reveals rules to guide rational design" .

Assembly members:

Assembly members:
entity_1, polymer, 11 residues, 3428.074 Da.
entity_2, polymer, 11 residues, 3547.162 Da.
entity_3, non-polymer, 382.435 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 32630 Superkingdom: not available Kingdom: not available Genus/species: synthetic construct

Experimental source:

Experimental source: Production method: recombinant technology Host organism: synthetic construct

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AUACXXACCUG
entity_2: GGAGUAAGUCU

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	147
1H chemical shifts	194

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	2
3	unit_3	3

Entities:

Entity 1, unit_1 11 residues - 3428.074 Da.

1

A

U

A

C

PSU

A

C

U

2

G

Entity 2, unit_2 11 residues - 3547.162 Da.

1

G

A

G

U

A

G

U

C

2

U

Entity 3, unit_3 - 382.435 Da.

1

ULR

Samples:

sample_1: RNA helix, unlableled, 1 ± 0.005 mM; RNA helix, unlableled, 1 ± 0.005 mM; SMN-CY, unlabeld, 1.5 ± 0.005 mM; MES, deuterated, 10 ± 0.001 mM; sodium chloride, unlabeld, 50 ± 0.001 mM

sample_conditions_1: ionic strength: 50 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1

Software:

Amber v22, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - data analysis, refinement

CYANA v3.98, Guntert, Mumenthaler and Wuthrich - structure calculation

CARA, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVIII 900 MHz
Bruker AVIII 700 MHz