BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34428

Title: Mouse RBM20 RRM domain in complex with AUCUUA RNA

Authors: Mackereth, C.; Upadhyay, S.

Citation: Mackereth, C.; Upadhyay, S.. "Structural basis of UCUU RNA motif recognition by splicing factor RBM20"  . ., .-..

Assembly members:
entity_1, polymer, 6 residues, 1757.153 Da.
entity_2, polymer, 112 residues, 12571.588 Da.

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: XUCUUA
entity_2: GAMAQRKGAGRVVHICNLPE GSCTENDVINLGLPFGKVTN YILMKSTNQAFLEMAYTEAA QAMVQYYQEKPAIINGEKLL IRMSTRYKELQLKKPGKNVA AIIQDIHSQRER

Data sets:
Data typeCount
13C chemical shifts501
15N chemical shifts123
1H chemical shifts854

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 6 residues - 1757.153 Da.

1   ADNUCUUA

Entity 2, entity_2 112 residues - 12571.588 Da.

1   GLYALAMETALAGLNARGLYSGLYALAGLY
2   ARGVALVALHISILECYSASNLEUPROGLU
3   GLYSERCYSTHRGLUASNASPVALILEASN
4   LEUGLYLEUPROPHEGLYLYSVALTHRASN
5   TYRILELEUMETLYSSERTHRASNGLNALA
6   PHELEUGLUMETALATYRTHRGLUALAALA
7   GLNALAMETVALGLNTYRTYRGLNGLULYS
8   PROALAILEILEASNGLYGLULYSLEULEU
9   ILEARGMETSERTHRARGTYRLYSGLULEU
10   GLNLEULYSLYSPROGLYLYSASNVALALA
11   ALAILEILEGLNASPILEHISSERGLNARG
12   GLUARG

Samples:

sample_1: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 400 uM; AUCUUA 480 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_2: Mouse RBM20 RRM domain, [U-99% 15N], 400 uM; AUCUUA 480 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_3: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 500 uM; AUCUUA 600 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_4: Mouse RBM20 RRM domain, [U-99% 2H; U-99% 15N], 300 uM; AUCUUA 290 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_5: Mouse RBM20 RRM domain, [U-99% 13C], 300 uM; AUCUUA 360 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_6: Mouse RBM20 RRM domain, [U-10% 13C; U-99% 15N], 450 uM; AUCUUA 560 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_7: Mouse RBM20 RRM domain, [U-99% 13C; U-99% 15N], 500 uM; AUCUUA 600 uM; sodium phosphate 20 mM; sodium chloride 50 mM

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_5isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC CTsample_6isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_5isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_5isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
2D 1H-1H TOCSYsample_4isotropicsample_conditions_1
2D x2-filtered 1H-1H NOESYsample_7isotropicsample_conditions_2
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
2D x2-filtered 1H-1H NOESYsample_5isotropicsample_conditions_1
3D (H)C(CO)NH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_6isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

Sparky, Goddard - chemical shift assignment

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

NMR spectrometers:

  • Bruker Neo 700 MHz
  • Bruker AVANCE III 800 MHz

Related Database Links: