BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34258

Title: Solution structure of FUS-ZnF bound to UGGUG   PubMed: 30581145

Authors: Loughlin, F.; Allain, F.

Citation: Loughlin, F.; Lukavsky, P.; Kazeeva, T.; Reber, S.; Hock, E.; Colombo, M.; Von Schroetter, C.; Pauli, P.; Clery, A.; Muhlemann, O.; Polymenidou, M.; Ruepp, M.; Allain, F.. "The Solution Structure of FUS Bound to RNA Reveals a Bipartite Mode of RNA Recognition with Both Sequence and Shape Specificity."  Mol. Cell 73, 490-504 (2019).

Assembly members:
entity_1, polymer, 41 residues, 4528.011 Da.
entity_2, polymer, 5 residues, 1602.992 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: GPLGSGQQRAGDWKCPNPTC ENMNFSWRNECNQCKAPKPD G
entity_2: UGGUG

Data typeCount
13C chemical shifts155
15N chemical shifts47
1H chemical shifts297

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22
3entity_33

Entities:

Entity 1, entity_1 41 residues - 4528.011 Da.

1   GLYPROLEUGLYSERGLYGLNGLNARGALA
2   GLYASPTRPLYSCYSPROASNPROTHRCYS
3   GLUASNMETASNPHESERTRPARGASNGLU
4   CYSASNGLNCYSLYSALAPROLYSPROASP
5   GLY

Entity 2, entity_2 5 residues - 1602.992 Da.

1   UGGUG

Entity 3, entity_3 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: FUS-ZnF, [U-99% 13C; U-99% 15N], 0.8 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 1.0 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_2: FUS-ZnF, [U-99% 13C; U-99% 15N], 0.8 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 1.0 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_3: FUS-ZnF, [U-99% 15N], 1.0 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 0.9 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_4: FUS-ZnF, [U-99% 13C; U-99% 15N], 1.0 mM; RNA (5'-R(*UP*GP*GP*UP*G)-3') 0.9 mM; sodium phosphate 20 mM; beta-mercaptoethanol 1 mM

sample_conditions_1: ionic strength: 20 mM; pH: 6.5; pressure: 1 Pa; temperature: 303 K

sample_conditions_2: ionic strength: 20 mM; pH: 6.5; pressure: 1 Pa; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_4isotropicsample_conditions_1
2D 13C F2-filtered NOESYsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_4isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_4isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

CANDID, Herrmann, Guntert and Wuthrich - peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

AMBER, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker AvanceIII 700 MHz
  • Bruker AvanceIII 600 MHz

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