BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34199

Title: Specific phosphorothioate substitution within domain 6 of a group II intron ribozyme leads to changes in local structure and metal ion binding   PubMed: 29218637

Authors: Erat, M.; Besic, E.; Oberhuber, M.; Johannsen, S.; Sigel, R.

Citation: Erat, M.; Besic, E.; Oberhuber, M.; Johannsen, S.; Sigel, R.; Erat, M.; Zerbe, O.; Fox, T.; Sigel, R.. "Specific phosphorothioate substitution within domain 6 of a group II intron ribozyme leads to changes in local structure and metal ion binding."  J. Biol. Inorg. Chem. 23, 167-177 (2018).

Assembly members:
entity_1, polymer, 27 residues, 8800.562 Da.

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GGAGCGGGGGXGXAAACCXA XCGCXCC

Data sets:
Data typeCount
1H chemical shifts203

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 27 residues - 8800.562 Da.

1   GGAGCGGGGG
2   U37GU37AAACCU37A
3   U37CGCU37CC

Samples:

sample_2: alpha-thio-D6-27 0.7 mM

sample_1: alpha-thio-D6-27 2 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.7 pD; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 50 mM; pH: 6.7; pressure: 1 atm; temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance 600 MHz

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