BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34100

Title: NMR solution structure of the TSL2 RNA hairpin

Authors: Garcia-Lopez, A.; Wacker, A.; Tessaro, F.; Jonker, H.; Richter, C.; Comte, A.; Berntenis, N.; Schmucki, R.; Hatje, K.; Sciarra, D.; Konieczny, P.; Fournet, G.; Faustino, I.; Orozco, M.; Artero, R.; Goekjian, P.; Metzger, F.; Ebeling, M.; Joseph, B.; Schwalbe, H.; Scapozza, L.

Citation: Garcia-Lopez, A.; Wacker, A.; Tessaro, F.; Jonker, H.; Richter, C.; Comte, A.; Berntenis, N.; Schmucki, R.; Hatje, K.; Sciarra, D.; Konieczny, P.; Fournet, G.; Faustino, I.; Orozco, M.; Artero, R.; Goekjian, P.; Metzger, F.; Ebeling, M.; Joseph, B.; Schwalbe, H.; Scapozza, L.. "Rational targeting of RNA structure in SMN2 transcripts reverses Spinal Muscular Atrophy molecular phenotypes"  Nat. Commun. ., .-..

Assembly members:
entity_1, polymer, 19 residues, 6048.626 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: AUUCCUUAAAUUAAGGAGU

Data sets:
Data typeCount
13C chemical shifts67
15N chemical shifts4
1H chemical shifts150

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 19 residues - 6048.626 Da.

1   AUUCCUUAAA
2   UUAAGGAGU

Samples:

sample_1: EDTA 0.1 mM; RNA (19-MER) 0.6 mM; sodium phosphate 50 mM

sample_2: EDTA 0.1 mM; RNA (19-MER) 0.6 mM; sodium phosphate 50 mM

sample_3: EDTA 0.1 mM; RNA (19-MER), [U-98% 13C; U-96-98% 15N]-ADE, 0.6 mM; sodium phosphate 50 mM

sample_conditions_1: ionic strength: 74 mM; pH: 6.4; pressure: 1 atm; temperature: 298 K

sample_conditions_2: ionic strength: 71 mM; pH: 6.4; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
2D 1H-1H NOESYsample_1isotropicsample_conditions_2
3D 1H-13C NOESYsample_3isotropicsample_conditions_1
2D 12C-filtered 1H-1H NOESYsample_3isotropicsample_conditions_1
2D 13C-editted 1H-1H NOESYsample_3isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_3isotropicsample_conditions_1
2D 1H-13C HSQC sugarsample_3isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1

Software:

ARIA v1.2 HJ development version, Linge, O'Donoghue and Nilges - refinement

CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - structure calculation

SPARKY v3.114, Kneller and Goddard - chemical shift assignment, data analysis, peak picking

TOPSPIN, Bruker Biospin - processing

TOPSPIN v3.2, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 900 MHz

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