BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 34099

Title: Structure of Tau(292-319) bound to F-actin   PubMed: 29215007

Authors: Fontela, Y.; Kadavath, H.; Zweckstetter, M.

Citation: Cabrales Fontela, Y.; Kadavath, H.; Biernat, J.; Riedel, D.; Mandelkow, E.; Zweckstetter, M.. "Multivalent cross-linking of actin filaments and microtubules through the microtubule-associated protein Tau."  Nat. Commun. 8, 1981-1981 (2017).

Assembly members:
entity_1, polymer, 28 residues, 2928.364 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: GSKDNIKHVPGGGSVQIVYK PVDLSKVT

Data sets:
Data typeCount
1H chemical shifts32

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 28 residues - 2928.364 Da.

1   GLYSERLYSASPASNILELYSHISVALPRO
2   GLYGLYGLYSERVALGLNILEVALTYRLYS
3   PROVALASPLEUSERLYSVALTHR

Samples:

sample_1: Tau(292-319) 800 uM; F-actin 27 uM; sodium phosphate 50 mM

sample_2: sodium phosphate 50 mM; Tau(254-290) 800 uM

sample_conditions_2: pH: 6.8; pressure: 1 atm; temperature: 278 K

sample_conditions_1: pH: 6.8; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H NOESYsample_2isotropicsample_conditions_2
2D 1H-1H TOCSYsample_2isotropicsample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

SPARKY, Goddard - chemical shift assignment, peak picking

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AvanceIII 900 MHz
  • Bruker AvanceIII 800 MHz

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