BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 34080

Title: hnRNP A1 RRM2 in complex with 5'-UCAGUU-3' RNA   PubMed: 28650318

Authors: Barraud, P.; Allain, F.

Citation: Beusch, Irene; Barraud, Pierre; Moursy, Ahmed; Clery, Antoine; Allain, Frederic Hai-Trieu. "Tandem hnRNP A1 RNA recognition motifs act in concert to repress the splicing of survival motor neuron exon 7"  Elife 6, e25736-e25736 (2017).

Assembly members:
Heterogeneous nuclear ribonucleoprotein A1, polymer, 102 residues, 11630.084 Da.
RNA UCAGUU, polymer, 6 residues, 1853.133 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
Heterogeneous nuclear ribonucleoprotein A1: SQRPGAHLTVKKIFVGGIKE DTEEHHLRDYFEQYGKIEVI EIMTDRGSGKKRGFAFVTFD DHDSVDKIVIQKYHTVNGHN CEVRKALSKQEMASASSSQR GR
RNA UCAGUU: UCAGUU

Data sets:
Data typeCount
13C chemical shifts293
15N chemical shifts92
1H chemical shifts654

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 102 residues - 11630.084 Da.

1   SERGLNARGPROGLYALAHISLEUTHRVAL
2   LYSLYSILEPHEVALGLYGLYILELYSGLU
3   ASPTHRGLUGLUHISHISLEUARGASPTYR
4   PHEGLUGLNTYRGLYLYSILEGLUVALILE
5   GLUILEMETTHRASPARGGLYSERGLYLYS
6   LYSARGGLYPHEALAPHEVALTHRPHEASP
7   ASPHISASPSERVALASPLYSILEVALILE
8   GLNLYSTYRHISTHRVALASNGLYHISASN
9   CYSGLUVALARGLYSALALEUSERLYSGLN
10   GLUMETALASERALASERSERSERGLNARG
11   GLYARG

Entity 2, entity_2 6 residues - 1853.133 Da.

1   UCAGUU

Samples:

sample_1: RNA UCAGUU 1.0 g/L; hnRNP A1 RRM2, [U-99% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_2: RNA UCAGUU 1.0 mM; hnRNP A1 RRM2, [U-99% 13C; U-99% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_3: RNA UCAGUU 1.0 mM; hnRNP A1 RRM2, [U-99% 15N], 1.0 mM; D2O 100%

sample_4: RNA UCAGUU 1.0 mM; hnRNP A1 RRM2, [U-99% 13C; U-99% 15N], 1.0 mM; D2O 100%

sample_conditions_1: ionic strength: 10 mM; pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_4isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_4isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D CBCA(CO)NHsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HCc(CO)NH TOCSYsample_2isotropicsample_conditions_1
3D hCC(CO)NH TOCSYsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D F1eF3f 13C-filtered/edited NOESYsample_4isotropicsample_conditions_1
2D F1fF2f 13C-filtered NOESYsample_4isotropicsample_conditions_1
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1

Software:

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

ProcheckNMR, Laskowski and MacArthur - data analysis

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

  • Bruker AvanceIII 500 MHz
  • Bruker AvanceIII 600 MHz
  • Bruker AvanceIII 750 MHz
  • Bruker AvanceIII 700 MHz
  • Bruker Avance 900 MHz

Related Database Links: