BMRB Entry 30973

Title:
Solution structure of 7SK stem-loop 1 with HIV-1 Tat Finland Arginine Rich Motif
Deposition date:
2021-12-02
Original release date:
2022-09-08
Authors:
Pham, V.; Gao, M.; D'Souza, V.
Citation:

Citation: Pham, V.; Gao, M.; Meagher, J.; Smith, J.; D'Souza, V.. "A structure-based mechanism for displacement of the HEXIM adapter from 7SK small nuclear RNA"  Commun. Biol. 5, 819-819 (2022).
PubMed: 35970937

Assembly members:

Assembly members:
entity_1, polymer, 56 residues, 17986.635 Da.
entity_2, polymer, 17 residues, 2144.537 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts219

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11
2unit_22

Entities:

Entity 1, unit_1 56 residues - 17986.635 Da.

1   GGGAUCUGUC
2   ACCCCAUUGA
3   UCGCCAGUGG
4   CUGAUCUGGC
5   UGGCUAGGCG
6   GGUCCC

Entity 2, unit_2 17 residues - 2144.537 Da.

1   GLYILESERTYRGLYARGLYSLYSARGLYS
2   HISARGARGARGALAHISGLN

Samples:

sample_1: 7SK-SL1 0.5 ± 0.2 mM; Tat Fin 0.5 ± 0.2 mM; Phosphate 10 mM; NaCl 70 mM; EDTA 0.1 mM

sample_2: 7SK-SL1 0.5 ± 0.2 mM; Tat Fin 0.5 ± 0.2 mM; Phosphate 10 mM; NaCl 70 mM; EDTA 0.1 mM

sample_conditions_1: ionic strength: 80 mM; pH: 5.6; pressure: 100000 Pa; temperature: 298 K

sample_conditions_2: ionic strength: 80 mM; pH: 5.6; pressure: 100000 Pa; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

TopSpin, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - data analysis, processing, structure calculation

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker DMX 700 MHz