BMRB Entry 30866

Title:
Structure of the cryptic HMA domain of the human copper transporter ATP7A
Deposition date:
2021-02-21
Original release date:
2021-09-07
Authors:
Lee, W.; Uhlemann, E.; Tonelli, M.; Dmitriev, O.
Citation:

Citation: Uhlemann, E.; Lee, W.; Tonelli, M.; Dmitriev, O.. "At sixes and sevens: a cryptic domain in the metal binding chain of the human copper transporter ATP7A"  Biophys. J. 120, 4600-4607 (2021).
PubMed: 34461106

Assembly members:

Assembly members:
entity_1, polymer, 76 residues, 8367.748 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB12

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts141
15N chemical shifts66
1H chemical shifts66

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 76 residues - 8367.748 Da.

1   ALAGLYHISMETTHRASPTHRLEUPHELEU
2   THRVALTHRALASERLEUTHRLEUPROTRP
3   ASPHISILEGLNSERTHRLEULEULYSTHR
4   LYSGLYVALTHRASPILELYSILETYRPRO
5   GLNLYSARGTHRVALALAVALTHRILEILE
6   PROSERILEVALASNALAASNGLNILELYS
7   GLULEUVALPROGLULEUSERLEUASPTHR
8   GLYTHRLEUGLULYSLYS

Samples:

sample_1: HMA2A, [U-13C; U-15N], 0.35 ± 0.02 mM; HEPES 50 ± 1 mM; sodium chloride 150 ± 1 mM; TCEP 5 ± 0.2 mM

sample_conditions_1: ionic strength: 210 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

APES, Lee - peak picking

NMRFAM-SPARKY, Lee, Tonelli, Markley - chemical shift assignment

I-PINE, Lee, Bahrami, Dashti, Eghbalnia, Tonelli, Westler and Markley - chemical shift assignment

CS-ROSETTA, Shen, Vernon, Baker and Bax - structure calculation

PONDEROSA-C/S, Lee, Stark, Markley - structure calculation

AUDANA, Lee, Petit, Cornilescu, Stark, Markley - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

NMR spectrometers:

  • Bruker AVANCE III 900 MHz
  • Varian Uniform NMR System 600 MHz
  • Bruker AVANCE III 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks