BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30626

Title: Solution structure of conotoxin MiXXVIIA   PubMed: 28984021

Authors: Daly, N.; Dekan, Z.; Jin, A.; Alewood, P.

Citation: Jin, A.; Dekan, Z.; Smout, M.; Wilson, D.; Dutertre, S.; Vetter, I.; Lewis, R.; Loukas, A.; Daly, N.; Alewood, P.. "Conotoxin phi-MiXXVIIA from the Superfamily G2 Employs a Novel Cysteine Framework that Mimics Granulin and Displays Anti-Apoptotic Activity."  Angew. Chem. Int. Ed. Engl. 56, 14973-14976 (2017).

Assembly members:
entity_1, polymer, 33 residues, 3422.695 Da.

Natural source:   Common Name: Soldier cone   Taxonomy ID: 69564   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus miles

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: EDCGSDCMPCGGECCCEPNS CIDGTCHHESSPN

Data sets:
Data typeCount
13C chemical shifts2
15N chemical shifts15
1H chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 33 residues - 3422.695 Da.

1   GLUASPCYSGLYSERASPCYSMETPROCYS
2   GLYGLYGLUCYSCYSCYSGLUPROASNSER
3   CYSILEASPGLYTHRCYSHISHISGLUSER
4   SERPROASN

Samples:

sample_1: phi-MiXXVIIA 1 mM

sample_2: phi-MiXXVIIA 1 mM

sample_conditions_1: ionic strength: 0 mM; pH: 5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

TopSpin, Bruker Biospin - collection

CcpNmr Analysis, CCPN - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

NMR spectrometers:

  • Bruker Avance 600 MHz

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