BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30454

Title: Tamapin mutant DP30

Authors: del Rio Portilla, F.; Melchor Meneses, C.; Titaux Delgado, G.; Mayorga Flores, M.

Citation: Mayorga Flores, M.; Toledano Magana, Y.; Melchor Meneses, C.; Lopez Giraldo, A.; Titaux Delgado, G.; Garcia Ramos, J.; Cano Sanchez, P.; Araiza Olivera, D.; Galindo Murillo, R.; del Rio Portilla, F.. "Cytotoxic activity of blocker scorpion toxins over cancer cells which constitutively express SK2 and SK3 potassium channels"  . ., .-..

Assembly members:
entity_1, polymer, 30 residues, 3373.071 Da.

Natural source:   Common Name: Eastern Indian scorpion   Taxonomy ID: 34647   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mesobuthus tamulus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
entity_1: AFCNLRRCELSCRSLGLLGK CIGEECKCVY

Data sets:
Data typeCount
1H chemical shifts201

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 30 residues - 3373.071 Da.

1   ALAPHECYSASNLEUARGARGCYSGLULEU
2   SERCYSARGSERLEUGLYLEULEUGLYLYS
3   CYSILEGLYGLUGLUCYSLYSCYSVALTYR

Samples:

sample_1: Tamapin mutant DP30 1.0 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

AMBER v16, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure calculation

CARA v1.8, Keller and Wuthrich - chemical shift assignment, peak picking

NMRPipe v2014, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 500.0 MHz

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