BMRB Entry 30423

Title:
Solution NMR structure of human BOK
Deposition date:
2018-03-01
Original release date:
2018-05-04
Authors:
Royappa, G.; Zheng, J.; Moldoveanu, T.
Citation:

Citation: Zheng, Janet; Grace, Christy; Guibao, Cristina; McNamara, Dan; Llambi, Fabien; Wang, Yue-Ming; Chen, Taosheng; Moldoveanu, Tudor. "Intrinsic Instability of BOK Enables Membrane Permeabilization in Apoptosis."  Cell Rep. 23, 2083-2094 (2018).
PubMed: 29768206

Assembly members:

Assembly members:
entity_1, polymer, 158 residues, 17186.006 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli 536

Data sets:
Data typeCount
13C chemical shifts535
15N chemical shifts163
1H chemical shifts1145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 158 residues - 17186.006 Da.

1   METSERPROTHRASPLYSGLULEUVALALA
2   GLNALALYSALALEUGLYARGGLUTYRVAL
3   HISALAARGLEULEUARGALAGLYLEUSER
4   TRPSERALAPROGLUARGALAALAPROVAL
5   PROGLYARGLEUALAGLUVALALAALAVAL
6   LEULEUARGLEUGLYASPGLULEUGLUMET
7   ILEARGPROSERVALTYRARGASNVALALA
8   ARGGLNLEUHISILESERLEUGLNSERGLU
9   ARGVALVALTHRASPALAPHELEUALAVAL
10   ALAGLYHISILEPHESERALAGLYILETHR
11   TRPGLYLYSVALVALSERLEUTYRALAVAL
12   ALAALAGLYLEUALAVALASPALAVALARG
13   GLNALAGLNPROALAMETVALHISALALEU
14   VALASPALALEUGLYGLUPHEVALARGLYS
15   THRLEUALATHRTRPLEUARGARGARGGLY
16   GLYTRPTHRASPVALLEULYSCYS

Samples:

sample_1: hBOK, [U-99% 13C; U-99% 15N], 0.5 mM; NaCl 150 mM

sample_conditions_1: ionic strength: 150 mM; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
3D-15N-NOESYsample_1isotropicsample_conditions_1
3D-13C-NOESYsample_1isotropicsample_conditions_1
3D-NOESY-arosample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - refinement, structure calculation

CARA v1.9, Keller and Wuthrich - chemical shift assignment, peak picking

TOPSPIN v3.5, Bruker Biospin - collection

NMR spectrometers:

  • Bruker AvanceII 600 MHz
  • Bruker AvanceIII 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks