BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30406

Title: Solution NMR Structure of Conotoxin G117 from Conus geographus

Authors: Armstrong, D.; Rosengren, K.

Citation: Armstrong, D.; Jin, A.-H., .; Lewis, R.; Alewood, P.; Rosengren, K.. "Solution NMR Structure of Conotoxin G117 from Conus geographus"  To be Published ., .-..

Assembly members:
entity_1, polymer, 32 residues, 3344.945 Da.

Natural source:   Common Name: Geography cone   Taxonomy ID: 6491   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus geographus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity_1: CAVTHEKCSDDYDCCGSLCC VGICAKTIAPCK

Data sets:
Data typeCount
13C chemical shifts66
15N chemical shifts29
1H chemical shifts170

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 32 residues - 3344.945 Da.

1   CYSALAVALTHRHISGLULYSCYSSERASP
2   ASPTYRASPCYSCYSGLYSERLEUCYSCYS
3   VALGLYILECYSALALYSTHRILEALAPRO
4   CYSLYS

Samples:

sample_1: conotoxin G117 1 mg/mL

sample_2: conotoxin G117 1 mg/mL

sample_conditions_1: ionic strength: 0 M; pH: 5.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H ECOSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

CYANA v3.96, Guntert, Mumenthaler and Wuthrich - structure calculation

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links: