BMRB Entry 30398
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR30398
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lan, Lan; Xing, Minli; Kashipathy, Maithri; Douglas, Justin; Gao, Philip; Battaile, Kevin; Hanzlik, Robert; Lovell, Scott; Xu, Liang. "Crystal and solution structures of human oncoprotein Musashi-2 N-terminal RNA recognition motif 1" Proteins 88, 573-583 (2020).
PubMed: 31603583
Assembly members:
entity_1, polymer, 115 residues, 13154.963 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
entity_1: MHHHHHHSTSVDLGTENLYF
QSNAGKMFIGGLSWQTSPDS
LRDYFSKFGEIRECMVMRDP
TTKRSRGFGFVTFADPASVD
KVLGQPHHELDSKTIDPKVA
FPRRAQPKMVTRTKK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 477 |
| 15N chemical shifts | 107 |
| 1H chemical shifts | 742 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 115 residues - 13154.963 Da.
| 1 | MET | HIS | HIS | HIS | HIS | HIS | HIS | SER | THR | SER | ||||
| 2 | VAL | ASP | LEU | GLY | THR | GLU | ASN | LEU | TYR | PHE | ||||
| 3 | GLN | SER | ASN | ALA | GLY | LYS | MET | PHE | ILE | GLY | ||||
| 4 | GLY | LEU | SER | TRP | GLN | THR | SER | PRO | ASP | SER | ||||
| 5 | LEU | ARG | ASP | TYR | PHE | SER | LYS | PHE | GLY | GLU | ||||
| 6 | ILE | ARG | GLU | CYS | MET | VAL | MET | ARG | ASP | PRO | ||||
| 7 | THR | THR | LYS | ARG | SER | ARG | GLY | PHE | GLY | PHE | ||||
| 8 | VAL | THR | PHE | ALA | ASP | PRO | ALA | SER | VAL | ASP | ||||
| 9 | LYS | VAL | LEU | GLY | GLN | PRO | HIS | HIS | GLU | LEU | ||||
| 10 | ASP | SER | LYS | THR | ILE | ASP | PRO | LYS | VAL | ALA | ||||
| 11 | PHE | PRO | ARG | ARG | ALA | GLN | PRO | LYS | MET | VAL | ||||
| 12 | THR | ARG | THR | LYS | LYS |
Samples:
sample_1: MSI2-RRM1, [U-90% 15N], 0.5 mM; NaCl 150 mM
sample_2: MSI2-RRM1, [U-95% 13C; U-90% 15N], 0.7 mM; NaCl 150 mM
sample_conditions_1: ionic strength: 150 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_2 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_2 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_2 | isotropic | sample_conditions_1 |
| 3D HNCACO | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_2 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-COSY | sample_2 | isotropic | sample_conditions_1 |
Software:
CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure calculation
CcpNMR, CCPN - chemical shift assignment, peak picking
NMRView, Johnson, One Moon Scientific - peak picking
TOPSPIN v2.1pl6, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker AvanceIII 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks